Journal of Capital Medical University ›› 2006, Vol. 27 ›› Issue (1): 70-73.

• 基础研究 • Previous Articles     Next Articles

Microarray Profiling of Inflammatory Gene Expression in Hypoxia-/Hypothermia-Treated Human Cerebral Endothelial Cells

Cui Hong1,2, Zhang Wandong2, Jamie Hutchison3, Danica Stanimirovic2   

  1. 1. Department of Pediatrics, Beijing Friendship Hospital, Capital University of Medical Sciences;2. Institute For BiologicSciences, National Research Council of Canada;3. The Children's Hospital of Eastern Ontario, PICU, Canada
  • Received:2004-10-09 Revised:1900-01-01 Online:2006-02-24 Published:2006-02-24

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Abstract: Objective To investigate the inflammatory gene expression in hypoxia-/hypothermia-treated human cerebral endothelial cells(HCEC).Methods Two groups were used: post hypoxic hypothermia and intra hypoxic hypothermia.For the experiments,the culture medium for HCEC was changed to M199(serum-free,low glucose) before the cells subject to hypoxia/hypothermia.Total RNA were then isolated and reverse-transcribed to cDNA for microarray experiments.Results The results showed that: 1) IL-1R1、IL-1RL1,IL-1β,IL-11,IRF2,IGFBP2,IGFBP3 and CSF1 were up-regulated,IL-1RN,IL-6,IFNGR2,CSF3R,SELL,ICAM3,PECAM 1and SELE were down-regulated at certain time-points.IFI30 was up-regulated during hypoxic hypothermia,but down-regulated after hypoxic hypothermia treatment.TNFRSF7 was down-regulated during hypothermia only.2) More genes were down-regulated by hypoxia for 4 h at 32 ℃ than that at 37 ℃.Conclusion Some inflammatory genes are up-regulated during hypoxia,such as IL-1β,IL1RL1 and IL-1R1.The up-regulated inflammatory genes such as IL-11 may be related to protective response to hypoxia.

Key words: cDNA microarray, hypoxia, mild hypothermia, human cerebral endothelial cells

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