The Effects of Hypoxia on Polarity of Hepatocytes Cultured in Vitro

Journal of Capital Medical University ›› 2005, Vol. 26 ›› Issue (4): 451-453.

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The Effects of Hypoxia on Polarity of Hepatocytes Cultured in Vitro

Zhang Xianjie¹, Sun Jiabang², Song Maomin¹, Yuan Huisheng¹, Duan Zhongping³

1. Department of General Surgery, Beijing Tiantan Hospital, Capital University of Medical Sciences;2. Department of General Surgery, Xuanwu Hospital, Capital University of Medical Sciences;3. Center of Rtificial Liver Support, Beijing You'an Hospital, Capital University of Medical Sciences

Received:2005-04-06 Revised:1900-01-01 Online:2005-08-24 Published:2005-08-24

Abstract

Abstract:

Objective To investigate the effects of hypoxia on polarity of hepatocytes cultured in vitro.Methods Sandwich-structural hepatocytes were used to study the effects of hypoxia on polarity of cultured hepatocytes. Expression of specific plasma protein, detensity of cytosolic Ca²⁺ and the morphology was observed before and after hypoxia.Results Both bile canaliculia-like structures and the distribution of specific plasma membrane protein were damaged in hypoxia treated hepatocytes. The detensity of cytosolic Ca²⁺ was increased in hypoxic hepatocytes.Conclusion Hypoxia induces significant disturbance of cell polarization in hepatocytes.

Key words: hepatocyte, polarity, in vitro, hypoxia

CLC Number:

R657.3

Zhang Xianjie;Sun Jiabang;Song Maomin;Yuan Huisheng;Duan Zhongping. The Effects of Hypoxia on Polarity of Hepatocytes Cultured in Vitro[J]. Journal of Capital Medical University, 2005, 26(4): 451-453.

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The Effects of Hypoxia on Polarity of Hepatocytes Cultured in Vitro

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