Neurotoxicity of HIV-1 envelope protein gp120 V3 loop

doi:10.3969/j.issn.1006-7795.2014.01.019

Abstract

Abstract:

Objective To investigate the neurotoxicity of human immunodeficiency virus-1(HIV-1) envelope protein gp120 in vitro. Methods Primary mouse cortical neuronal cultures were firstly established for neurotoxicity analysis. Terminal dUTP nick end-labeling (TUNEL) assay and lactate dehydrogenase (LDH) leakage assay were used to analyze neuronal apoptosis induced by recombinant gp120 V3 loop polypeptide. Further, Western blotting was used to detect the levels of apoptosis associated proteins bcl-2 and bax under gp120 V3 loop exposure. Results Recombinant gp120 V3 loop polypeptide was found to induce neuronal apoptosis and inhibit neurite outgrowth in a concentration dependent manner, which was associated with low expression of bcl-2 proteins. Conclusion HIV-1 envelope protein gp120 has neurotoxicity.

Key words: envelope protein gp120, neurotoxicity, V3 loop, apoptosis

CLC Number:

R512.91

Zhang Zhili, Chen Binggang, Jiang Taiyi, Qiao Luxin, Wu Hao, Chen Dexi, Zhang Yulin. Neurotoxicity of HIV-1 envelope protein gp120 V3 loop[J]. Journal of Capital Medical University, 2014, 35(1): 87-91.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2014.01.019

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2014/V35/I1/87

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