Effect of Bmi-1 on apoptosis of human fetal striatum derived neural stem cells

doi:10.3969/j.issn.1006-7795.2015.01.020

Journal of Capital Medical University ›› 2015, Vol. 36 ›› Issue (1): 103-110.doi: 10.3969/j.issn.1006-7795.2015.01.020

Previous Articles Next Articles

Effect of Bmi-1 on apoptosis of human fetal striatum derived neural stem cells

Zhao Chunsong^1,2, Zou Haiqiang³, Yan Xiaoming⁴, Chen Ling⁵, Wang Jiayin^1,2, Guan Yunqian^1,2, Zhang Y. Alex^1,2

1. Department of Cell Biology, Beijing Institute of Geriatrics, Xuanwu Hospital, Capital Medical University, Beijing 100053, China;
2. Key Laboratory of Neurodegeneration, Ministry of Education, Beijing 100053, China;
3. Department of Neurology, The General Hospital of Guangzhou Military Command in Guangzhou, Guangzhou 510010, China;
4. Department of Function Neurosurgery, Xuanwu Hospital, Capital Medical University, Beijing 100053, China;
5. Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, Beijing 100053, China

Received:2014-09-11 Online:2015-02-21 Published:2015-01-31
Supported by:
This study was supported by National Program on Key Basic Research Project (2012CBA01300);National High Technology Research and Development Program of China (2011AA020106); Natural Science Foundation of Beijing (7102078); Beijing Nova Program of Science and Technology (2009B22); Beijing Municipal Science and Technology Commission (Z111107067311033); Key Project of Beijing Municipal Education Commission Science and Technology Program(KZ201310025024).

Abstract

Abstract: Objective To explore the effect of Bmi-1 on the apoptosis of human fetal striatum derived neural stem cell (NSC). Methods The NSC was isolated from the striatum of 16-18 week fetus suffered from accidental spontaneous abortion, cultured and passaged 3-5 times in vitro. Bmi-1RNA interference and rescue (over-expression of Bmi-1) experiments were performed by lentivirus. The effects of Bmi-1 on the apoptosis of NSC were then observed.Results The Bmi-1 transcription of human striatum derived NSCs was decreased significantly by RNAi and was reversed by rescue experiments(overexpression of Bmi-1). Correspondingly, both at the time points of 2h and 3d after passaging, the RNAi of Bmi-1 increased the apoptosis significantly. Conclusion Given human fetal striatum derived NSCs, Bmi-1 inhibited their apoptosis in vitro. In the cases studying the effect of Bmi-1 on the self-renewal, proliferation of NSC, the anti-apoptosis effect of Bmi-1 should be taken into consideration.

Key words: neural stem cell, apoptosis, Bmi-1, oncogene

CLC Number:

R392.2

Zhao Chunsong, Zou Haiqiang, Yan Xiaoming, Chen Ling, Wang Jiayin, Guan Yunqian, Zhang Y. Alex. Effect of Bmi-1 on apoptosis of human fetal striatum derived neural stem cells[J]. Journal of Capital Medical University, 2015, 36(1): 103-110.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2015.01.020

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2015/V36/I1/103

References

[1] Gage F H. Mammalian neural stem cells[J]. Science, 2000, 287(5457): 1433-1438.
[2] Doetsch F, Alvarez-Buylla A. Network of tangential pathways for neuronal migration in adult mammalian brain[J]. Proc Natl Acad Sci USA, 1996, 93(25): 14895-14900.
[3] Schmidt-Hieber C, Jonas P, Bischofberger J. Enhanced synaptic plasticity in newly generated granule cells of the adult hippocampus[J]. Nature, 2004, 429(6988):184-187.
[4] Molofsky A V, Slutsky S G, Joseph N M,et al. Increasing p16INK4a expression decreases forebrain progenitors and neurogenesis during ageing[J]. Nature, 2006, 443(7110):448-452.
[5] Krishnamurthy J, Ramsey M R, Ligon K L, et al. p16INK4a induces an age-dependent decline in islet regenerative potential[J]. Nature, 2006, 443(7110):453-457.
[6] Janzen V, Forkert R, Fleming H E, et al. Stem-cell ageing modified by the cyclin-dependent kinase inhibitor p16INK4a[J]. Nature, 2006, 443(7110): 421-426.
[7] Molofsky A V, Pardal R, Iwashita T, et al. Bmi-1 dependence distinguishes neural stem cell self-renewal from progenitor proliferation[J]. Nature, 2003, 425(6961): 962-967.
[8] Wang Y, Guan Y, Wang F, et al. Bmi-1 regulates self-renewal, proliferation and senescence of human fetal neural stem cells in vitro[J]. Neurosci Lett, 2010, 476(2): 74-78.
[9] Zaidi H A, Kosztowski T, DiMeco F, et al. Origins and clinical implications of the brain tumor stem cell hypothesis[J]. J Neurooncol, 2009, 93(1): 49-60.
[10] Cui H, Hu B, Li T, et al. Bmi-1 is essential for the tumorigenicity of neuroblastoma cells[J]. Am J Pathol, 2007, 170(4): 1370-1378.
[11] Wiederschain D, Chen L, Johnson B, et al. Contribution of polycomb homologues Bmi-1 and Mel-18 to medulloblastoma pathogenesis[J]. Mol Cell Biol, 2007, 27(13): 4968-4979.
[12] 张红,刘庆华,周丽,等. 骨髓增生异常综合征原癌基因Bmi-1表达的检测[J]. 中华肿瘤防治杂志, 2014,21(3):211-213.
[13] Wu Z, Min L, Chen D, et al. Overexpression of BMI-1 promotes cell growth and resistance to cisplatin treatment in osteosarcoma[J]. PLoS One, 2011, 6(2): e14648.
[14] Lee K, Adhikary G, Balasubramanian S, et al. Expression of Bmi-1 in epidermis enhances cell survival by altering cell cycle regulatory protein expression and inhibiting apoptosis[J]. J Invest Dermatol, 2008, 128(1): 9-17.
[15] Liu ZG, Liu L, Xu LH, et al. Bmi-1 induces radioresistance in MCF-7 mammary carcinoma cells[J]. Oncol Rep, 2012, 27(4): 1116-1122.
[16] Li J, Gong LY, Song LB, et al. Oncoprotein Bmi-1 renders apoptotic resistance to glioma cells through activation of the IKK-nuclear factor-kappaB Pathway[J]. Am J Pathol, 2010, 176(2): 699-709.
[17] Jakob S, Corazza N, Diamantis E, et al. Detection of apoptosis in vivo using antibodies against caspase-induced neo-epitopes[J]. Methods, 2008, 44(3): 255-261.
[18] Boonman Z, Isacson O. Apoptosis in neuronal development and transplantation: role of caspases and trophic factors[J]. Exp Neurol, 1999, 156(1): 1-15.

Effect of Bmi-1 on apoptosis of human fetal striatum derived neural stem cells

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	Zhang Ying, Liu Chaonan, Shi Honglin, Liu Fang, Chen Dexi, Shi Hongbo. Transcriptome analysis of lipid metabolization-related differential genes mediated by P53 apoptosis-stimulating protein 2 in alcoholic liver disease [J]. Journal of Capital Medical University, 2023, 44(1): 85-92.
[2]	Liu Zhibin, Jin Song, Niu Yinong. The effects and mechanism of phenylethyl isothiocyanate on cell proliferation and apoptosis in BPH-1 cells [J]. Journal of Capital Medical University, 2021, 42(6): 961-966.
[3]	An Songlin, Ma Ling, Zhao Yue, Li Yan. Effects of major facilitator superfamily domain containing 2A on the cell proliferation, apoptosis and invasion of human hepatocellular carcinoma [J]. Journal of Capital Medical University, 2021, 42(6): 1000-1006.
[4]	Cui Xing, Zhong Ping. Experimental study of anti miRNA-181a induces apoptosis via Mitophagy enhancing on Multiple Myeloma cells [J]. Journal of Capital Medical University, 2021, 42(1): 167-169.
[5]	Liu Yan, Chen Yujing, Chen Wenqiang, Huang Xiaobo. Effects of Yuanzhi decoction on cognitive function and apoptosis-related protein in hippocampus of rats with chronic cerebral hypoperfusion [J]. Journal of Capital Medical University, 2019, 40(3): 323-329.
[6]	Zhang Yichen, Cao Xi, Yang Jinkui. Effect of G protein-coupled receptor MAS on hepatic glycolipid metabolism [J]. Journal of Capital Medical University, 2019, 40(3): 409-416.
[7]	Lu Jing, Shen Han, Cheng Cheng, Liu Jingyi, Zhu Xiaorong, Xie Rongrong, Yuan Mingxia, Yang Jinkui. Effect of human ether-α-go-go related gene channels on hepatic endoplasmic reticulum stress and apoptosis [J]. Journal of Capital Medical University, 2019, 40(1): 45-52.
[8]	Song Lini, Cao Xi, Liu Wei, Yang Jinkui. Effects of angiotensin converting enzyme 2 on apoptosis of hepatocytes [J]. Journal of Capital Medical University, 2018, 39(2): 223-229.
[9]	Peng Shiguang, Li Na, Lan Zulian, Li Li, He Yanling. Overexpression of promyelocytic leukaemia gene correlated with p53 and bcl-2 family in psoriatic peripheral lymphocytes [J]. Journal of Capital Medical University, 2017, 38(5): 645-649.
[10]	Guo Yixian, Zhang Lanfang, Sun Xuejing, Wan Suigui, Xia Changqing. Alloantigen immune tolerance and its mechanism in mice immunized with the ultraviolet radiation immature dendritic cells [J]. Journal of Capital Medical University, 2017, 38(3): 431-438.
[11]	Shi Tingting, Yang Fangyuan, Cao Xi, Lu Jing, Xie Rongrong, Yuan Mingxia, Yang Jinkui. Angiotensin converting enzyme 2 improves insulin secretion in islets [J]. Journal of Capital Medical University, 2017, 38(2): 151-155.
[12]	Mu Lingyun, Li Jinxia, Zhang Qiuyun, Chen Yu, Gao Lianyin, Du Yuqiong. Effects of Jieduan Niwan Formula on the c-Jun,p-JNK of liver tissue in Acute-on-Chronic liver failure rats [J]. Journal of Capital Medical University, 2017, 38(2): 282-288.
[13]	Shi Wenjuan, Qi Zhifeng, Liu Kejian. Normobaric hyperoxia protect cerebral ischemia/reperfusion injured rats by downregulating voltage-dependent anion channel protein [J]. Journal of Capital Medical University, 2017, 38(1): 42-46.
[14]	Yang Weiwei, Li Xuran, Li Xin, Li Xuying, Yu Shun. Expression of neuronal hemoglobin alleviates α-synuclein-induced apoptosis in MES23.5 cells [J]. Journal of Capital Medical University, 2016, 37(6): 799-805.
[15]	Guo Caixia, Jiang Xue, Zeng Xiangjun, Chen Buxing. Effect of STAT3 in soluble receptor for advanced glycation end-products inhibiting myocardial apoptosis induced by ischemia/reperfusion [J]. Journal of Capital Medical University, 2016, 37(1): 41-47.