Evaluation of biological characteristics of two u87 glioma cell lines after STR typing technique

doi:10.3969/j.issn.1006-7795.2018.01.014

Abstract

Abstract: Objective To detect cell biological characteristics of the two U87 glioma cell lines[U-87 MG Glioblastoma-Astrocytoma Human in American Type Culture Collection (ATCC) database and U-87 MG in Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) database] which were identified by short tandem repeats (STR) typing technique, and to evaluate the possibility of two cells for follow-up research.Methods By CCK-8 method, scratch test, transwell assay, and combined with the morphological characteristics were done to investigate the proliferation, migration and invasion ability of the two tumor cell lines.Results (1) CCK-8 method:the cell growth curve showed no statistical difference in proliferation ability(P>0.05). (2) Scratch wound test:the migration ability of two cell lines is no statistical difference (P>0.05). (3) Transwell assay:the migration ability of two cell lines is no significant difference (P> 0.05), and the invasive ability of U-87 MG in DSMZ database is significantly higher than that of U-87 MG Glioblastoma-AstrocytomaHuman in ATCC database (P<0.05).Conclusion Both two U87 cell lines represent the biological properties of brain gliomas and can be used for subsequent experimental studies. The proliferation ability and migration ability of the two cell lines are not significantly different, and the difference of invasive ability is statistically significant. We can choose the appropriate cell line for experimental purposes.

Key words: glioma, U87 cell lines, biological characteristics

CLC Number:

R739.41

Zhang Wenli, Kong Fanhong, He Lu, Dong Chengya, Wang Yajie. Evaluation of biological characteristics of two u87 glioma cell lines after STR typing technique[J]. Journal of Capital Medical University, 2018, 39(1): 74-78.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2018.01.014

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2018/V39/I1/74

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