Effect of estrogen on the proliferation and nude mouse tumorigenicity of endometrial carcinoma cells by mediating the expression of HOTAIR

doi:10.3969/j.issn.1006-7795.2019.06.016

Abstract

Abstract: Objective To investigate the effect of estrogen on the proliferation and nude mouse tumorigenicity of endometrial carcinoma cells by mediating the expression of HOTAIR. Methods Lentivirus-mediated interference of HOTAIR expression shHOTAIR and negative control shNC were constructed and transfected into Ishikawa cells, the expression of HOTAIR was detected by qRT-PCR. Four groups were set up:control group (Ishikawa cells), E2 group (E2+Ishikawa cells), E2+shNC group (E2+shNC cells) and E2+shHOTAIR group (E2+shHOTAIR cells). qRT-PCR was used to detect the effect of E2 on the expression of HOTAIR in the four groups. The xenograft tumor model of nude mice treated with E2 and four groups Ishikawa cells of different expression of HOTAIR was constructed. The tumor formation time, the tumor formation rate and the growth curve of the xenograft tumor were recorded. The xenograft tumor was removed, whose volume and weight were measured. The pathological examination of the xenograft tumor was performed. Results The stable transfected cell lines with lentivirus-mediated interference of HOTAIR expression shHOTAIR and negative control shNC were successfully constructed, and the expression of HOTAIR was effectively inhibited (P<0.001). The relative expression of HOTAIR in E2 group was significantly higher than that of control group (P<0.001), and E2+shHOTAIR group was significantly lower than E2+shNC group (P<0.001). The xenograft tumor model of nude mice treated with E2 and four groups Ishikawa cells of different expression of HOTAIR was successfully constructed. The comparison of tumorigenic ability was as follows:E2 group>control group,E2+shNC group>E2+shHOTAIR group. The final volume (P<0.001) and final weight (P<0.001) of the xenograft tumor in E2 group were significantly higher than those in control group, and the final volume (P<0.01) and final weight (P<0.001) in E2+shHOTAIR group were significantly lower than those in E2+shNC group. The HE staining of the xenograft tumor was consistent with the pathological characteristics of endometrial carcinoma cells. Conclusion Estrogen promotes the proliferation and nude mouse tumorigenicity of endometrial carcinoma cells by up-regulating the expression of HOTAIR, which is expected to provide a new target for the treatment of endometrial carcinoma.

Key words: endometrial carcinoma, estrogen, HOTAIR, long non-coding RNAs

CLC Number:

R737.33

Ma Xulan, Xia Di, Wang Huixiao, Jiang Ziwen, Dai Yinmei. Effect of estrogen on the proliferation and nude mouse tumorigenicity of endometrial carcinoma cells by mediating the expression of HOTAIR[J]. Journal of Capital Medical University, 2019, 40(6): 894-900.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2019.06.016

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2019/V40/I6/894

References

[1] Bray F, Ferlay J, Soerjomataram I, et al. Global cancer statistics 2018:GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin, 2018,68(6):394-424.
[2] Bokhman J V. Two pathogenetic types of endometrial carcinoma[J]. Gynecol Oncol, 1983,15(1):10-17.
[3] Huang J, Ke P, Guo L, et al. Lentivirus-mediated RNA interference targeting the long noncoding RNA HOTAIR inhibits proliferation and invasion of endometrial carcinoma cells in vitro and in vivo[J]. Int J Gynecol Cancer, 2014,24(4):635-642.
[4] 王慧霄,代荫梅.干涉lncRNAs HOTAIR对人子宫内膜癌Ishikawa细胞的影响[J].中国妇幼保健,2018,33(11):2572-2574.
[5] Knauss J L, Sun T. Regulatory mechanisms of long noncoding RNAs in vertebrate central nervous system development and function[J]. Neuroscience, 2013,235:200-214.
[6] Gutschner T, Diederichs S. The hallmarks of cancer:A long non-coding RNA point of view[J]. RNA Biol, 2012,9(6):703-719.
[7] Rinn J L, Kertesz M, Wang J K, et al. Functional demarcation of active and silent chromatin domains in human HOX loci by noncoding RNAs[J]. Cell, 2007,129(7):1311-1323.
[8] Collina F, Aquino G, Brogna M, et al. LncRNA HOTAIR up-regulation is strongly related with lymph nodes metastasis and LAR subtype of triple negative breast cancer[J]. J Cancer, 2019,10(9):2018-2024.
[9] Guo X, Xiao H, Guo S, et al. Long noncoding RNA HOTAIR knockdown inhibits autophagy and epithelial-mesenchymal transition through the Wnt signaling pathway in radioresistant human cervical cancer HeLa cells[J]. J Cell Physiol, 2019,234(4):3478-3489.
[10] Yang L, Peng X, Li Y, et al. Long non-coding RNA HOTAIR promotes exosome secretion by regulating RAB35 and SNAP23 in hepatocellular carcinoma[J]. Mol Cancer, 2019,18(1):78.
[11] Zhan S, Wang K, Song Y, et al. Long non-coding RNA HOTAIR modulates intervertebral disc degenerative changes via Wnt/β-catenin pathway[J]. Arthritis Res Ther, 2019,21(1):201.
[12] Zhou W, He X, Chen Z, et al. LncRNA HOTAIR-mediated Wnt/β-catenin network modeling to predict and validate therapeutic targets for cartilage damage[J]. BMC Bioinformatics, 2019,20(1):412.
[13] Tsai M C, Manor O, Wan Y, et al. Long noncoding RNA as modular scaffold of histone modification complexes[J]. Science, 2010,329(5992):689-693.
[14] Zhao X, Tian X. Knockdown of long noncoding RNA HOTAIR inhibits cell growth of human lymphoma cells by upregulation of miR-148b[J]. J Cell Biochem, 2019,120(8):12348-12359.
[15] Song Y, Wang R, Li L W, et al. Long non-coding RNA HOTAIR mediates the switching of histone H3 lysine 27 acetylation to methylation to promote epithelial-to-mesenchymal transition in gastric cancer[J]. Int J Oncol, 2019,54(1):77-86.
[16] Sun M Y, Zhu J Y, Zhang C Y, et al. Autophagy regulated by lncRNA HOTAIR contributes to the cisplatin-induced resistance in endometrial cancer cells[J]. Biotechnol Lett, 2017,39(10):1477-1484.
[17] Xue M, Chen L Y, Wang W J, et al. HOTAIR induces the ubiquitination of Runx3 by interacting with Mex3b and enhances the invasion of gastric cancer cells[J]. Gastric Cancer, 2018,21(5):756-764.
[18] Bhan A, Hussain I, Ansari K I, et al. Antisense transcript long noncoding RNA (lncRNA) HOTAIR is transcriptionally induced by estradiol[J]. J Mol Biol, 2013,425(19):3707-3722.
[19] 朱圣韬,孙秀静,李鹏,等.PHF8基因对食管鳞状细胞癌裸鼠移植瘤生长的影响[J].首都医科大学学报,2016,37(1):12-16.
[20] 叶园英,黄煜,颜莉莉,等.人子宫内膜癌裸鼠皮下移植瘤模型的建立[J].山东医药,2016,56(35):28-30.
[21] 邹路遥,林国志.17β-E2联合顺铂对人卵巢癌细胞裸鼠移植瘤的影响[J].中国现代医生,2018,56(4):39-42.