Abstract:

15 male adult Wistar rats were divided into experimental splenic deficiency group(10 rats)and normal control group(5 rats).Both groups of rats were fed under same conditions. In the experimental group,after administration of thubarb for 9 days,typical splenic deficiency symptoms were inducecd.The tail portions of pancreas of both groups were taken at 10 th day,and treated routinely for paraffin sections.Sternberger's PAP method was used to demonstrate the A-cells(glucagon cells),B-cells(insulin cells)and D-cells(somatostatin cells)in order to investigate their changes.Based on the intensity and content of the immunoreactive substances in the cells.In comparison,we found that in the experimental group the secretion of insulin elevated(P<0.05)and the secretion of glucagon and somatostatin declined obviously(P<0.05).The significance of these changes were also descussed.

Key words: experimental splenic deficiency, immunohistochemistry, pancreatic islet