Journal of Capital Medical University ›› 1995, Vol. 16 ›› Issue (2): 95-98.

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Studies on Alkaline Phosphatase(ALP) and Leucine Aminopeptidase(LNAse) Activity in Mouse Embryonal and Postnatal Developing Testis

Zhu Dingshou, Lu Xin, Shi Xiaolin, Weng Jing, Xu Qing   

  1. Department of Reproductive Medicine, Capital Institute of Medicine
  • Received:1994-01-11 Revised:1900-01-01 Online:1995-04-15 Published:1995-04-15

Abstract:

Two enzymes,ALP and LNAse,were demostrated by histochemistry in the embryonal and postnatal developing testes of mouse. ALP was shown clearly to have positional change in testes from embryo to maturity.In embryo,ALP was found in the primordial germ cells.But by day 10 after birth,none of the germ cells showed positive reaction for ALP.From day 10 on,the basement membrane of seminiferous tubules in postnatal developing testes showed positive reaction for ALP.The positive reaction became stronger by day 20.LNAse was identified in primordial germ cells and Sertoli's cells of embryonal testes.In the postnatal developing testes LNAse were also positive.LNAse and ALP may be important enzymes for the proliferation and differentiation of germinal cells in mouse testes.

Key words: Alkaline phosphatase, Leucine Aminopeptidase, histochemistry, mouse testis

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