Study on the Expression of Ki-67 and Apoptosis in the Pancreas Cancer Cell Line Bxpc-3

Abstract

Abstract: To test the realtionship between Ki-67 index and apoptosis in the pancreas cancer cell line Bxpc-3, apoptosis was inducted by 1,25(OH)₂D₃ in the pancreas cancer cell line. We compared the cell number by MTT, determined quantitatively apoptosis cells by TUNEL, detected the expression of Ki-67 index by immunohistochemical. Results suggested that the expression of the Ki-67 index was decreased during apoptosis induced by 10^-8mol/L 1,25(OH)₂D₃ in Bxpc-3 cell line. There was negative correlation between the apoptosis and the expression of Ki-67 index. Comprehensive analysis of the apoptosis and Ki-67 index reflects the balance of proliferation and apoptosis.

Key words: 25-(OH)₂D₃, Bxpc-3, apoptosis, Ki-67

CLC Number:

Liu Shuang;Liu Dachuan;Li Fei;Sun Jiabang. Study on the Expression of Ki-67 and Apoptosis in the Pancreas Cancer Cell Line Bxpc-3[J]. Journal of Capital Medical University, 2003, 24(3): 307-309.

[1]	Li Yanglu, Ruan Xiangyan, Du Juan, Cheng Jiaojiao, Jin Fengyu, Gu Muqing, Li Yanqiu, Alfred O. Mueck. Impact of cryopreservation and thawing on human ovarian tissue [J]. Journal of Capital Medical University, 2025, 46(4): 594-600.
[2]	Li Yue, Chen Junyu, Chang Chunlei, Zhang Jing. Investigation on the mechanism of Huatan Anshen Decoction to improve neuronal apoptosis based on NLRP3/IL-18/NF-κB pathway [J]. Journal of Capital Medical University, 2025, 46(3): 487-495.
[3]	Gou Dongyun, Liang Jiayi, Guo Ziyu, Yan Fangyuan, Wang Shuaixiang , Qu Shuo, Gao Yan, Zhang Yujia, Ma Xiaowei. The p38 MAPK signaling pathway mediates the reduction of apoptosis, inflammation and oxidative stress levels in AD mice by cerebroprotein [J]. Journal of Capital Medical University, 2024, 45(6): 1062-1070.
[4]	Liu Xiu, Li Wencai. Preliminary study on the effect of UNC0379 on the proliferation of glioma cells [J]. Journal of Capital Medical University, 2023, 44(5): 741-746.
[5]	. A view of sulforaphane interference with microtubules and mitochondrial kinetics in anti-cancer mechanisms [J]. Journal of Capital Medical University, 2023, 44(3): 363-369.
[6]	Yang Yifan, Wang Yamei. Expression and mechanism of apoptosis-inducing factor 2 gene in breast cancer [J]. Journal of Capital Medical University, 2023, 44(2): 248-257.
[7]	Zhang Ying, Liu Chaonan, Shi Honglin, Liu Fang, Chen Dexi, Shi Hongbo. Transcriptome analysis of lipid metabolization-related differential genes mediated by P53 apoptosis-stimulating protein 2 in alcoholic liver disease [J]. Journal of Capital Medical University, 2023, 44(1): 85-92.
[8]	Liu Zhibin, Jin Song, Niu Yinong. The effects and mechanism of phenylethyl isothiocyanate on cell proliferation and apoptosis in BPH-1 cells [J]. Journal of Capital Medical University, 2021, 42(6): 961-966.
[9]	An Songlin, Ma Ling, Zhao Yue, Li Yan. Effects of major facilitator superfamily domain containing 2A on the cell proliferation, apoptosis and invasion of human hepatocellular carcinoma [J]. Journal of Capital Medical University, 2021, 42(6): 1000-1006.
[10]	Cui Xing, Zhong Ping. Experimental study of anti miRNA-181a induces apoptosis via Mitophagy enhancing on Multiple Myeloma cells [J]. Journal of Capital Medical University, 2021, 42(1): 167-169.
[11]	Zhang Lingyan, Ruan Xiangyan, Cai Guiju, Gu Muqing, Alfred O. Mueck. Effect of tamoxifen on Ki-67 expression in breast tumor tissue of nude mice transfected with progesterone receptor membrane component 1 [J]. Journal of Capital Medical University, 2019, 40(4): 533-538.
[12]	Liu Yan, Chen Yujing, Chen Wenqiang, Huang Xiaobo. Effects of Yuanzhi decoction on cognitive function and apoptosis-related protein in hippocampus of rats with chronic cerebral hypoperfusion [J]. Journal of Capital Medical University, 2019, 40(3): 323-329.
[13]	Zhang Yichen, Cao Xi, Yang Jinkui. Effect of G protein-coupled receptor MAS on hepatic glycolipid metabolism [J]. Journal of Capital Medical University, 2019, 40(3): 409-416.
[14]	Lu Jing, Shen Han, Cheng Cheng, Liu Jingyi, Zhu Xiaorong, Xie Rongrong, Yuan Mingxia, Yang Jinkui. Effect of human ether-α-go-go related gene channels on hepatic endoplasmic reticulum stress and apoptosis [J]. Journal of Capital Medical University, 2019, 40(1): 45-52.
[15]	Song Lini, Cao Xi, Liu Wei, Yang Jinkui. Effects of angiotensin converting enzyme 2 on apoptosis of hepatocytes [J]. Journal of Capital Medical University, 2018, 39(2): 223-229.

Study on the Expression of Ki-67 and Apoptosis in the Pancreas Cancer Cell Line Bxpc-3

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments