Method for Culture of the Neural Stem Cells Isolated from the Hippocampus in Human Embryo

Journal of Capital Medical University ›› 2004, Vol. 25 ›› Issue (2): 144-146.

• 论著·基础研究 • Previous Articles Next Articles

Method for Culture of the Neural Stem Cells Isolated from the Hippocampus in Human Embryo

Zhao Chunli¹, Sun Xiaohong¹, Lu Qiang¹, Liu Yujun¹, Li Min², Jiang Chuantao¹, Yang Hui¹, Xu Qunyuan¹

1. Beijing Institute for Neuroscience, Beijing Center for Neural Regeneration and Repairing, Capital University of Medical Sciences;2. Xuanwu Hospital, Capital University of Medical Sciences

Received:2003-12-15 Revised:1900-01-01 Online:2004-04-15 Published:2004-04-15

Abstract

Abstract: The aim was to establish a method for culture of neural stem cells (NSCs) isolated from the hippocampus in human embryo, to prevent differentiation of them and to explore a suitable time and technique for passaging. NSCs were isolated from the hippocampus of 16-weeks old human embryo by using serum-free mediums with different conditions. The numbers of neurospheres in different mediums were counted and compared. Results: The suitable culture conditions and passage intervals were found for the culture of NSCs. The results show that the NSCs can be remained in an indifferentiated situation under the anti-differentiation condition in vitro and also can be passaged continuously.

Key words: neural stem cells, culture, passage

CLC Number:

R322.81

Zhao Chunli;Sun Xiaohong;Lu Qiang;Liu Yujun;Li Min;Jiang Chuantao;Yang Hui;Xu Qunyuan. Method for Culture of the Neural Stem Cells Isolated from the Hippocampus in Human Embryo[J]. Journal of Capital Medical University, 2004, 25(2): 144-146.

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Method for Culture of the Neural Stem Cells Isolated from the Hippocampus in Human Embryo

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