An Animal Model of Spinal Cord Injury-induced Spontaneous Pain

Journal of Capital Medical University ›› 2005, Vol. 26 ›› Issue (6): 670-672.

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An Animal Model of Spinal Cord Injury-induced Spontaneous Pain

Dai Hong¹, Xu Shaoting², Xiao Zhongxin¹, Zhang Hong³, Xie Zuolei⁴, Zhang Lijun³, Chen Yingchao², Zhang Liren², Wang Yuanshen⁵, Dai Ke⁶

1. Department of Rehabilitation Medicine, School of Public Health and Family Medicine, Capital University of Medical(Sciences);2. Department of Orthopedics, Beijing Military General Hospital;3. Paraplegics Sanatorium of Tang Shan;4. (Department) of Physiology, Institute of Basic Medical Sciences, Peking Union Medical College;5. Beijing Institute of Neuroscience, Capital University of Medical Sciences;6. Department of neuropathology, Beijing Neurosurgical Institute

Received:2005-11-08 Revised:1900-01-01 Online:2005-12-24 Published:2005-12-24

Abstract

Abstract: Objective To establish a practical and reliable animal model of spontaneous pain after spinal injury.Methods 30 female Wistar rats were divided into 2 groups: G1 and G2.The injury was induced in L₂ segment of spinal cord using₃ Ncm (G1) and 2 Ncm (G2) by WADEmethod, respectively.SEP (somatosensory evoked potential) was recorded in L₁ before injury induction and 15 min after injury.The pain threshold of rats in trunk to mechanical gently pressure, gently tactile and rate of forepaws'withdrawing was assessed using von Frey hair just before and after 4 h, 8 h, 12 h, 16 h, 24 h injury everyday for about 10 months.Behavior activities of spontaneous pain were also determined everyday.Results Two groups exhibited typical allodynia phenomena.The pain threshold before and after injury has decreased significantly (P<0.05～0.01) .The obvious spontaneous pain could be observed.The proportion of serious spontaneous pain (autotomy) in G1 group is 50%, which was near to that of the clinical observations.Conclusion SCIof 3 Ncm in rat model by WADEmethod has an excellent repeatability. (It's) spontaneous pain symptom is near to that of CPin SCIpatients.The animal model will be useful for SCIpain studies.

Key words: spinal cord injury, spontaneous pain, animal model, WADE method

CLC Number:

R332

Dai Hong;Xu Shaoting;Xiao Zhongxin;Zhang Hong;Xie Zuolei;Zhang Lijun;Chen Yingchao;Zhang Liren;Wang Yuanshen;Dai Ke. An Animal Model of Spinal Cord Injury-induced Spontaneous Pain[J]. Journal of Capital Medical University, 2005, 26(6): 670-672.

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An Animal Model of Spinal Cord Injury-induced Spontaneous Pain

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