Abstract:

Objective To study the effect of Shenshuning mixture on phenotypic modulation of mesangial cell in glomerulosclerosis rats and its mechanism. Methods Rats models were induced and established by unilateral nephrectomy(Nx) and injection. Rats model of glomerulosclerosis was established by unilateral nephrectomy and intravenous injection of 0.2% Doxorubicin(5 mg/kg), rats were administered Shenshuning mixture 43.93 g/(kg·d), benazepril 10 mg/(kg·d), or distilled water respectively. Other 8 sham-operated rats as comparison. 8 weeks later, The expression of glomerularα-SMA and TGF-β₁ were studied by immunohistochemical staining, and the ratio of positive area/total glomerular capillary area(SMA/GT and TGF/GT)were analyzed with image analysis software. the changes of weight, 24 h proteinuria, plasma albumin, total serum proteim and blood urine nitrogen, creatinine clearance rate were examined in each group. Results Immunohistochemical staining of glomeruli showed that expression of α-SMA and TGF-β₁ in Shenshuning treated group and Benazepril treated group were significantly less than those of model group(P<0.01) and improved rats weights, attenuated 24 h urine protein, blood urine nitrogen and serum creatinine(P<0.01). The relatively analysis showed significant corelation of α-SMA and TGF-β₁ factors(r=0.637, P<0.01). Conclusion Shenshuning mixture can improve glomerulosclerosis by inhibiting phenotypic modulation of mesangial cells and TGF-β₁ expression, attenuate 24 h proteinuria excretion, blood urea nitrogen and creatinine.

Key words: Shenshuning, glomerulosclerosis, phenotypic modulation, &alpha, -smooth muscle actin, transforming growth factor-&beta, ₁