Model and Mechanism of the Insulin Transport across Human Immortalized Oral Epithelial Cell Monolayers

Abstract

Abstract: Objective The purposes of this study are to evaluate the mechanism of delivering insulin with enhancer and additives on the in vitro cultured monolayer system of HIOEC(the human immortalized oral epithelial cell) lines.Methods The HIOECs formed continuous monolayers with polycarbonate filters of Transwell plate served to evaluate the transport of insulin.The transport studies were carried out at different conditions,with different penetration enhancers(HP-β-CD,chitosan,polyoxyethylene lauryl ether,egg lecithin,or oleic acid),and different additives(poly-L-lysine,colchicine).Results Under different conditions,the transport of insulin was not direction-,temperature-,and concentration-dependent.Conclusion The enhancing effects might be due to one or multiple factors:increasing the mucosal lipid fluidity,directly loosing the tight junction,and changing the steric conformation of protein.

Key words: insulin, enhancing mechanism, transport

CLC Number:

R587

Cui Chunying;Cui Guohui;Zhao Ming. Model and Mechanism of the Insulin Transport across Human Immortalized Oral Epithelial Cell Monolayers[J]. Journal of Capital Medical University, 2008, 29(3): 327-331.

[1]	Zhang Dongxue, Wen Zhen, Sun Yuyan, Jiang Tao. Association between hepatic insulin clearance,clinical characteristics and gut hormone in Chinese patients with newly diagnosed type 2 diabetes mellitus [J]. Journal of Capital Medical University, 2021, 42(5): 822-828.
[2]	Wang Jingshang, Yin Xiaodan, He Junqin, Wu Ying, Zhang Ying, Li Xianrui. Experimental study on establishment of polycystic ovary syndrome rat model with insulin resistance by letrozole combined with high-sugar and high-fat diet [J]. Journal of Capital Medical University, 2021, 42(3): 436-442.
[3]	Li Zhu, Li Nan, Liu Zhibo, Yang Zhi. Research on glycine cell uptake mechanisms [J]. Journal of Capital Medical University, 2021, 42(1): 15-20.
[4]	Li Qi, Lu Jing, Zhu Xiaorong, Xiong Fengran, Yang Jinkui. Generation and identification of Unc13 gene pancreatic beta cell knockout mice model [J]. Journal of Capital Medical University, 2020, 41(1): 14-20.
[5]	Chen Si, Zhou Cui, Yu Huiyan, Tao Lingwei, Wang Yushan, Xiao Rong. The influence of 27-hydroxycholesterolon cholesterol transport associated factor in co-cultured SH-SY5Y cells and C6 cells [J]. Journal of Capital Medical University, 2019, 40(2): 269-275.
[6]	Ping Hao, Ma Linxiang, Wang Mingshuai, Long Jun, Niu Yinong, Liu Yuexin, Xing Nianzeng. Expression and regulation of monocarboxylate transporters 4(MCT4) in prostate cancer [J]. Journal of Capital Medical University, 2019, 40(1): 59-64.
[7]	Ning Junyu, Jing Haiming, Du Hongju, Qi Lijuan, Gao Shan, Li Guojun. AMP-activated protein kinase inhibits insulin-like growth factor-1 signaling in vascular smooth muscle cells via regulating activation of extracellular signal-regulated kinases 1/2 [J]. Journal of Capital Medical University, 2019, 40(1): 101-105.
[8]	Zhang He, Gou Rongbin, Niu Hanxu, Sun Xiaohong, Xu Qunyuan, Duan Deyi. Neural cell differentiation in the brain of insulin-like growth factor binding protein 4 transgenic mouse [J]. Journal of Capital Medical University, 2018, 39(6): 910-916.
[9]	Jin Jing, Ruan Xiangyan, Hua Lin, Li Yanglu, Wang Lijuan, Wang Husheng, Zhao Yue, Alfred O. Mueck. Characteristics of obesity and glycolipid metabolism in polycystic ovary syndrome patients with diminished ovarian reserve [J]. Journal of Capital Medical University, 2018, 39(4): 505-511.
[10]	Lu Xingmeng, Xin Zhong. Ang (1-7) improves skeletal muscle lipid metabolism and insulin resistance by regulating endoplasmic reticulum stress [J]. Journal of Capital Medical University, 2018, 39(4): 532-536.
[11]	Jin He, Zhao Zhiwei, Wang Rong. Correlation between catch up growth and Alzheimer's disease [J]. Journal of Capital Medical University, 2017, 38(6): 831-834.
[12]	Yin Dongmei, Ruan Xiangyan, Zhao Yue, Alfred O. Mueck. Analysis of endocrine and metabolic characteristics in patients with polycystic ovary syndrome in Beijing [J]. Journal of Capital Medical University, 2017, 38(4): 498-503.
[13]	Zhu Lili, Chen Zhe, Liu Xin, Yu Na, Pan Qingrong. Relationships between OGTT 1-h plasma glucose levels and insulin sensitivity and pancreatic β cell function in subjects with normal glucose tolerance [J]. Journal of Capital Medical University, 2017, 38(4): 592-596.
[14]	Shi Tingting, Yang Fangyuan, Cao Xi, Lu Jing, Xie Rongrong, Yuan Mingxia, Yang Jinkui. Angiotensin converting enzyme 2 improves insulin secretion in islets [J]. Journal of Capital Medical University, 2017, 38(2): 151-155.
[15]	Lu Jing, Sheng Han, Cheng Cheng, Song Lini, Zhang Yichen, Xie Rongrong, Yuan Mingxia, Yang Jinkui. Human ether-α-go-go related gene channels improves glucose metabolism via anti-oxidative stress in HepG2 cells [J]. Journal of Capital Medical University, 2017, 38(2): 156-160.

Model and Mechanism of the Insulin Transport across Human Immortalized Oral Epithelial Cell Monolayers

PDF

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments