Abstract: Objective To apply a novel proteomic method to analyze differential protein expression in serum and biomarkers in pregnant women with preeclampsia. Methods Sera from five pregnant women complicated with severe preeclampsia and five healthy pregnant controls were collected and mixed respectively. separated protein. Each mixture was treated with hydrophilic-lipophilic balance(HLB) and peptide ligand library beads (PLLBs) to remove highly abundant proteins and thus enriched less abundant proteins; the proteins from the elute were quantitatively arrayed by a combination of 1D gel -LC/MS/MS.Results Twenty-eight proteins in HLB and 51 proteins in PLLB were differentially expressed between preeclampsia and healthy pregnant women including IL -17F and coruleplasmin.Conclusion The result demonstrated that peptide ligand library combined with 1D gel-LC-MS/MS analysis is an effective method to identify expressed proteins in serum and inflammatory factor may be involved in the etiology of preeclampsia.

Key words: preeclampsia, hydrophilic-lipophilic balance, peptide ligand library bead, inflammatory factor