Screening and analysis of differentially expressed genes related to breast cancer

doi:10.3969/j.issn.1006-7795.2013.01.001

Abstract

Abstract:

Objective To screen differentially expressed genes between breast cancer tissues and corresponding normal breast tissues by constructing a cDNA subtractive library with suppression subtractive hybridization (SSH) technique.Methods Fifteen pairs of breast cancer and corresponding normal breast tissues were studied to construct a cDNA subtractive library by SSH. Amplification of the library was carried out with E. coli strain DH5α. The cDNA was sequenced and analyzed with the relevant sequences in GenBank with Blast search after PCR. Results The subtracted cDNA library of differentially expressed genes of breast cancer was successfully constructed and 173 differentially expressed cDNA fragments were obtained. Homology analysis showed that 26 of them were tumor-associated genes in which 15 genes were reported to be related with breast cancer.Conclusion The construction of cDNA library of differentially expressed genes of breast cancer provides a useful pathway for exploring the pathogensis of breast cancer and looking for the potential target genes for treating breast cancer.

Key words: suppression subtractive hybridization, breast cancer, differentially expressed genes, cDNA library

CLC Number:

R737.9

ZHANG Chun, LIU Jun, JIANG Hongchuan, HUANG Jingwei, QÜ Shiying, WANG Tie. Screening and analysis of differentially expressed genes related to breast cancer[J]. Journal of Capital Medical University, 2013, 34(1): 1-5.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2013.01.001

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2013/V34/I1/1

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