Expression and role of IL-23 in chronic rhinosinusitis

doi:10.3969/j.issn.1006-7795.2020.02.006

Abstract

Abstract: Objective To investigate the expression of interleukin-23(IL-23) in chronic rhinosinusitis and the regulation in T helper cell 17(Th-17) response. Methods Fifteen control subjects, 14 patients with chronic rhinosinusitis without nasal polyps(CRSsNP) and 37 patients with chronic rhinosinusitis with nasal polyps (CRSwNP) were recruited. Tissue samples were obtained from the inferior turbinates of control subjects, the ethmoid mucosa of patients with CRSsNP, and the nasal polyps(NP) of patients with CRSwNP, respectively; and the tissue homogenates were prepared. The concentrations of IL-23, myelo-peroxidase(MPO) and IL-17 in tissue homogenates were measured. The number of neutrophils in nasal tissue and the count and percent of neutrophils in peripheral blood were counted. Dispersed nasal polyp cells were prepared from fresh NP tissue and stimulated with IL-23 for 24h in vitro and the concentrations of Th17-related cytokines in the culture supernatants were measured. Results Compared to normal control, no change of IL-23 concentrations in tissue homogenates from CRSsNP and CRSwNP were observed. However, IL-23 concentration in IL-17+CRSwNP was significantly higher than that in the control and IL-17-CRSwNP. IL-23 concentration in tissue homogenates was positively correlated with tissue MPO concentration, but had no correlation with peripheral or tissue neutrophil number. IL-23 simulation promoted the production of IL-17 and IL-1β in dispersed nasal polyp cells, but the production of IL-8 and granulocyte colony stimulating factor(G-CSF) did not change. Conclusion IL-23 may play a role in IL-17+CRSwNP via increasing Th17 response.

Key words: chronic rhinosinusitis, interleukin-23(IL-23), T helper cell 17(Th17), neutrophil

CLC Number:

R765

Wang Min, Li Ying, Wang Xiangdong, Zhang Luo. Expression and role of IL-23 in chronic rhinosinusitis[J]. Journal of Capital Medical University, 2020, 41(2): 178-182.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2020.02.006

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2020/V41/I2/178

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