Research on glycine cell uptake mechanisms

doi:10.3969/j.issn.1006-7795.2021.01.003

Abstract

Abstract: Objective To study the quantitative relationship between the uptake of ¹⁸F-BF₃-Phe and the expression of large-neutral amino acid transporter-1(LAT-1), and to elucidate the uptake mechanism of ¹⁸F-BF₃-Phe in cancer cells. Methods ¹⁸F-BF₃-Phe uptake experiments was performed with different levels of LAT-1 transfected cells. The ¹⁸F-BF₃-Phe uptake by PC9, BXPC3, PC3, HEPG2 cells was assayed at different time points. Results The uptake of ¹⁸F-BF₃-Phe in LAT-1 transfected cells was linearly correlated with the increase in the proportion of LAT-1 transfection. The uptake of ¹⁸F-BF₃-Phe in PC9, BXPC3, PC3, and HepG2 tumor cells showed an increasing trend over time. The uptake of ¹⁸F-BF₃-Phe by PC9 and BXPC3 cells was significantly higher than the other two cell lines. Conclusion The uptake of ¹⁸F-BF₃-Phe by LAT-1 transfected cells was positively correlated with the expression of LAT-1 transporter, which provided an important theoretical basis for further in vivo experiments. ¹⁸F-BF₃-Phe was highly uptaken by a variety of tumor cells, showing potential in clinical translation of this probe in tumor imaging.

Key words: boramino acid, amino acid transporter, tumor, radiolabelling

CLC Number:

R817.1

Li Zhu, Li Nan, Liu Zhibo, Yang Zhi. Research on glycine cell uptake mechanisms[J]. Journal of Capital Medical University, 2021, 42(1): 15-20.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2021.01.003

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2021/V42/I1/15

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