Limb remote ischemic perconditioning combined with remote ischemic postconditioning promotes neurogenesis in rat models of ischemic stroke

doi:10.3969/j.issn.1006-7795.2023.01.009

Abstract

Abstract: Objective To explore comfirm the effect of limb remote ischemic per-conditioning (PerC) combined with post-conditioning (PostC) on neurogenesis after ischemic stroke, and to explore the effect of PerC combined with PostC on the rate-limiting enzyme of fatty acid β-oxidation (FAO): carnitine palmitoyl transferase 1A (CPT1A). Methods Adult male Sprague Dawley rats were subjected to middle cerebral artery occlusion (MCAO). Limb remote ischemic conditioning was applied 30 min after the MCAO model, followed by repeated short episodes of remote ischemic conditioning 24 h after reperfusion (post-conditioning). The neurogenesis in the subependymal ventricular zone(SVZ) region was analyzed with immunohistochemistry staining, and the expression of CPT1A was analyzed with enzyme linked immunosorbent assay(ELISA). Results PerC+PostC treatment significantly improved neurological function and increased the number of neural stem cells (NSCs) and the number of cells that migrated to the infarct area compared to the MCAO and PerC/PostC groups. Pearson correlation analysis showed that the number of neural stem cells had a negative correlation with neurological function. However, the number of apoptotic NSCs that migrated to the basal ganglia did not differ significantly between groups. Further mechanism studies showed that the treatment with PerC+PostC significantly increased the protein level of CPT1A. Conclusion PerC combined with PostC treatment can improve neurological function by increasing the number of NSCs. FAO of NSCs may be one of its mechanisms to promote the migration of NSCs.

Key words: ischemic stroke, limb remote ischemic conditioning, neurogenesis, fatty acid β-oxidation

CLC Number:

R743.3

Qin Linhui, Li Ning, Yang Yu, Yang Yong, Ren Changhong. Limb remote ischemic perconditioning combined with remote ischemic postconditioning promotes neurogenesis in rat models of ischemic stroke[J]. Journal of Capital Medical University, 2023, 44(1): 54-61.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2023.01.009

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2023/V44/I1/54

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