Amp-FLP Analysis on D5S436 Locus and Its Application to Linkage Analysis in Gene Diagnosis of Asthma

Journal of Capital Medical University ›› 1998, Vol. 19 ›› Issue (3): 237-240.

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Amp-FLP Analysis on D5S436 Locus and Its Application to Linkage Analysis in Gene Diagnosis of Asthma

Huang Kewu¹, Weng Xinzhi¹, Liu Jingzhong², Zhu Zhangling², Xiao Bai², Yan Mei², Zhang Hongyu¹, Wang Chen¹

Beijing Respiratory Diseases Research Center, Beijing Red Cross Chaoyang Hospital, Affiliate of Capital University of Medical Sciences

Received:1997-09-11 Revised:1900-01-01 Online:1998-07-15 Published:1998-07-15

Abstract

Abstract: To detect the polymorphsism of D5S436 locus by using amplified fragment length polymorphism(Amp-FLP), allele and genotype frequencies of the hightly polymorphic D5S436 locus were determined in 92 unrelated Chinese individuals. The amplified fragments were separated by denaturing polyacrylamide gel electrophoresis and the gel was silver stained. 8 alleles and 22 genotypes were observed in this population. The heterozygosity was 78.26%. The polymorphism information content(PIC)is 0.79. Amp-FLP genotyping linkage analyses were carried out in four asthma families, demonstrated that the locus was inherited according to Mendelian law and have a clear result. It is concluded that D5S436 can be used as a genetic marker of asthma in Chinese and maybe helpful in the gene diagnosis of asthma.

Key words: D5S436 locus, DNA polymorphism, asthma

CLC Number:

Huang Kewu;Weng Xinzhi;Liu Jingzhong;Zhu Zhangling;Xiao Bai;Yan Mei;Zhang Hongyu;Wang Chen. Amp-FLP Analysis on D5S436 Locus and Its Application to Linkage Analysis in Gene Diagnosis of Asthma[J]. Journal of Capital Medical University, 1998, 19(3): 237-240.

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Amp-FLP Analysis on D5S436 Locus and Its Application to Linkage Analysis in Gene Diagnosis of Asthma

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