Streptococcus pneumoniae infection at early stage of life affect the severity of asthma induced by exposure to the same bacterial antigens in adulthood

doi:10.3969/j.issn.1006-7795.2023.02.005

Abstract

Abstract: Objective To investigate the influence and mechanisms of infection with Streptococcus pneumoniae(SP)at early stage of life and late exposure to the same bacterial antigens on the occurrence and development of asthma.Methods Mice (one week old of BALB/c background) were infected with SP, then 5 weeks later challenged intranasally with inactivate SP bacteria and interleukin-33 (IL-33)+inactivated SP bacteria to establish model. Lung function and airways hyperresponsiveness (AHR), total cellular and differential counts in bronchoalveolar lavage fluid (BALF) and lungs were measured to evaluate the inflammatory cell infiltration and inflammatory changes in the airways/lungs.The expression levels of IgE and SP specific IgE and IgG in serum, as well as the expression levels of various cytokines in lung homogenates were detected with ELISA.The number of T helper cell (Th) subgroup (Th1, Th2, Th17) and innate lymphoid cells (ILC1, ILC2, ILC3) were measure with flow cytometry. Results Early-life airways infection with SP and repeated exposure to the same bacterial antigen alone in adulthood failed to induce asthma-like pathological changes. In the presence of IL-33, early-life airways infection with SP and repeated exposure to the same bacterial antigen attenuated asthma-like pathological changes, including the dereased AHR, inflammatory cellular infiltration of the airways, secretion of mucus and smooth muscle hyperplasia in lung tissue, serum concentrations of IgE and concentrations of Th2-type cytokines, numbers of activated Th1, Th2, Th17 and ILC1 cells in the lung tissues, but an increased IL-10 expression, compared with those mice without SP infection in early life. Conclusion Early-life airways infection with SP attenuted the severity of asthma induced by exposure to the same bacterial antigens in adulthood.

Key words: early birth infections, Streptococcus pneumoniae, interleukin-33 (IL-33), bacterial antigens, asthma

CLC Number:

R392

Peng Dan, Pang Jie, Shi Yifan, Cui Lele, Xu Yingjie, Li Yan, Cui Ye, Chen Yan, Yuan Huihui, Qin Xiaofeng, Lyu Zhe, Wang Wei, Sun Ying. Streptococcus pneumoniae infection at early stage of life affect the severity of asthma induced by exposure to the same bacterial antigens in adulthood[J]. Journal of Capital Medical University, 2023, 44(2): 212-220.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2023.02.005

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2023/V44/I2/212

References

［1］Nordengrün M, Michalik S, Vlker U, et al. The quest for bacterial allergens［J］. Int J Med Microbiol, 2018, 308(6):738－750.
［2］Murrison L B, Brandt E B, Myers J B, et al. Environmental exposures and mechanisms in allergy and asthma development［J］. J Clin Invest, 2019, 129(4):1504－1515.
［3］Li C D, Du X N, Huang Q, et al. Repeated exposure to inactivated Streptococcus pneumoniae induces asthma-like pathological changes in mice in the presence of IL-33［J］. Cell Immunol, 2021, 369: 104438.
［4］Peng D, Shi Y F, Pang J, et al. Early-life infection of the airways with Streptococcus pneumoniae exacerbates HDM-induced asthma in a murine model［J］. Cell Immunol, 2022, 376: 104536.
［5］Renz H, Skevaki C. Early life microbial exposures and allergy risks: opportunities for prevention［J］. Nat Rev Immunol, 2021, 21(3):177－191.
［6］Renz H, Herz U. The bidirectional capacity of bacterial antigens to modulate allergy and asthma［J］. Eur Respir J, 2002, 19(1):158－171.
［7］Shi T, Ooi Y, Zaw E M, et al. Association between respiratory syncytial virus-associated acute lower respiratory infection in early life and recurrent wheeze and asthma in later childhood［J］. J Infect Dis, 2020, 222(Suppl 7):S628-S633.
［8］Culley F J, Pollott J, Openshaw P J M. Age at first viral infection determines the pattern of T cell-mediated disease during reinfection in adulthood［J］. J Exp Med, 2002, 196(10):1381－1386.
［9］Bisgaard H, Hermansen M N, Buchvald F, et al. Childhood asthma after bacterial colonization of the airway in neonates［J］. N Engl J Med, 2007, 357(15):1487－1495.
［10］Tersjrvi J T, Toivonen L, Vuononvirta J, et al. TLR4 polymorphism, nasopharyngeal bacterial colonization, and the development of childhood asthma: a prospective birth-cohort study in finnish children［J］. Genes (Basel), 2020, 11(7):768.
［11］Weiser J N, Ferreira D M, Paton J C. Streptococcus pneumoniae: transmission, colonization and invasion［J］. Nat Rev Microbiol, 2018, 16(6):355－367.
［12］Larsen S B, Cowley C J, Sajjath S M, et al. Establishment, maintenance, and recall of inflammatory memory［J］. Cell Stem Cell, 2021, 28(10):1758－1774.e8.
［13］Liew F Y, Girard J P, Turnquist H R. Interleukin-33 in health and disease［J］. Nat Rev Immunol, 2016, 16(11):676－689.
［14］Alvarez F, Fritz J H, Piccirillo C A. Pleiotropic effects of IL-33 on CD4+ T cell differentiation and effector functions［J］. Front Immunol, 2019, 10: 522.
［15］Naik S, Larsen S B, Gomez N C, et al. Inflammatory memory sensitizes skin epithelial stem cells to tissue damage［J］. Nature, 2017, 550(7677):475－480.
［16］Kim C W, Yoo H J, Park J H, et al. Exogenous interleukin-33 contributes to protective immunity via cytotoxic T-cell priming against mucosal influenza viral infection［J］. Viruses, 2019, 11(9):840.
［17］Lei Y, Boinapally V, Zoltowska A, et al. Vaccination against IL-33 inhibits airway hyperresponsiveness and inflammation in a house dust mite model of asthma［J］. PLoS One, 2015, 10(7):e0133774.
［18］Hung L Y, Tanaka Y, Herbine K, et al. Cellular context of IL-33 expression dictates impact on anti-helminth immunity［J］. Sci Immunol, 2020, 5(53):eabc6259.

[1]	Chen Yan, Zhang Xulong, Xu Jiangnan, Jia Yufeng, Sun Ying. Advances in modern immunology in infectious diseases, allergic diseases, tumors, and neurological disorders [J]. Journal of Capital Medical University, 2023, 44(2): 179-185.
[2]	Wang Yi, Ding Yuezhong, Xu Jiangnan. Research progress of follicular helper T cells and their roles in antibody-mediated lung transplant rejection [J]. Journal of Capital Medical University, 2023, 44(2): 186-195.
[3]	Zhang Hanxiao, Duan Luo, Zhang Muzhi, Zhang Ruoyang, Zhao Liuyinuo, Zhou Ruonan, Li Yunqi, Lyu Zhe, Wang Jingjing, Yuan Huihui, Cui Ye, Sun Ying, Wang Wei. Immune characteristics of short-term chronic obstructive pulmonary disease model [J]. Journal of Capital Medical University, 2023, 44(2): 203-211.
[4]	Li Ziyi, Wang Xi, Liang Pu. Progresses on the effect of estrogen on innate immune cells [J]. Journal of Capital Medical University, 2023, 44(2): 351-357.
[5]	Bai Ruojing, Lyu Shiyun, Hua Wei, Wu Hao, Dai Lili. Construction of LILRB2 overexpression lentivirus vector and THP-1-LILRB2 stable transformation cell [J]. Journal of Capital Medical University, 2023, 44(1): 93-98.
[6]	Li Mo, Han Deqiang, Zhao Yu, Chen Zhiguo. Harnessing cell therapy as a therapeutic strategy to fight disease——Professor Chen Zhiguo [J]. Journal of Capital Medical University, 2020, 41(5): 778-782.
[7]	Chen Rongjuan, Chen Qirui, Xu Jiangnan, Ding Yuezhong. Effects to obliterative bronchiolitis by blocking CD40-CD40L pathway in the mouse lung transplantation [J]. Journal of Capital Medical University, 2018, 39(2): 258-264.
[8]	Chen Chen, Meng Yan. Ang Ⅱ activates dendritic cell to produce immune function [J]. Journal of Capital Medical University, 2017, 38(5): 709-714.
[9]	Zhang Yue, Li Yun, Zhu Jinxia, Zhao Wenming. Expression and cellular distribution of dopamine receptors in the rat colonic submucosal plexus [J]. Journal of Capital Medical University, 2017, 38(3): 411-416.
[10]	Yuan Linjie, Chen Shihao, An Gao, Huang Qiong, Yi Dawei, Li Yan, Lyu Zhe, Wang Jingjing, Huang Kewu, Wang Wei, Sun Ying. Changes of immune cells and their subsets in lungs of interleukin (IL)-33-induced allergen-independent murine model of asthma [J]. Journal of Capital Medical University, 2016, 37(5): 561-567.
[11]	Huang Aihua, Zhang Pingping, Zhang Bin, Ma Buqing, Guan Yunqian, Zhou Yidan. Replicative senescence reduced the efficacy of human fetal bone marrow-derived mesenchymal stem cell intravenous treatment of cerebral infarction rats [J]. Journal of Capital Medical University, 2015, 36(6): 865-874.
[12]	Zhao Chunsong, Zou Haiqiang, Yan Xiaoming, Chen Ling, Wang Jiayin, Guan Yunqian, Zhang Y. Alex. Effect of Bmi-1 on apoptosis of human fetal striatum derived neural stem cells [J]. Journal of Capital Medical University, 2015, 36(1): 103-110.
[13]	Feng Xueting, Zhang Chenguang, Zhang Min, Yue Zhixia, Xing Tianyu, Li Shentao, Ding Wei. Application of HaloTag technology in the intracellular imaging and quantitative analysis of alpha-synuclein [J]. Journal of Capital Medical University, 2014, 35(3): 324-330.
[14]	WANG Min, YANG Jun, SHANG Jun, ZHANG Luo. Expression of tumor necrosis factor-α-inducible protein-2 in a mouse model of allergic rhinitis and its relationship with angiogenesis [J]. Journal of Capital Medical University, 2013, 34(6): 790-794.
[15]	LI Xiaobo, GU Jianjuan, ZHAO Chunsong, WANG Shuyan, GUAN Yunqian, CHEN Ling, ZHANG Yu. Lentiviral vector mediated hLPP-3 expression in human umbilical cord derived mesenchymal stem cells [J]. Journal of Capital Medical University, 2013, 34(6): 872-878.