α-Synuclein promotes hydrogen peroxide-induced apoptosis of dopaminergic neuronal cells

doi:10.3969/j.issn.1006-7795.2011.06.013

Journal of Capital Medical University ›› 2011, Vol. 32 ›› Issue (6): 771-776.doi: 10.3969/j.issn.1006-7795.2011.06.013

• 帕金森病的发病机制研究 • Previous Articles Next Articles

α-Synuclein promotes hydrogen peroxide-induced apoptosis of dopaminergic neuronal cells

LI Ying¹, LIU Guang-wei¹, LI Xin¹, LI Yao-hua¹, YANG Hui², YU Shun¹

1. Department of Neurobiology, Xuanwu Hospital, Capital Medical University;Key Laboratory of Neurodegenerative Diseases, Ministry of Education;Laboratory of Molecular Diagnosis, Beijing Geriatric Medical Research Center, Beijing, 100053, China;2. Beijing Institute for Neuroscience, Capital Medical University, Beijing 100069, China

Received:2011-10-16 Revised:1900-01-01 Online:2011-12-21 Published:2011-12-21

Abstract

Abstract: Objective To investigate the role of α-synuclein (α-syn) in hydrogen peroxide(H₂O₂)-induced apoptosis of dopaminergic neuronal cells. Methods α-Syn overexpression cell model was established by transient transfection of plasmids pEGFP-α-syn or pcDNA-α-syn into MES23.5 dopaminergic neuronal cells. The cells were treated with H₂O₂(200 μmol/L) to induce cell apoptosis. Hoechst 33258 and AO/PI fluorescent labeling were used to examine apoptosis. Mitochondrial probe Mitotracker was used to label mitochondria. Results H₂O₂ increased translocation of α-syn into mitochondria and nuclei and induced apoptosis of both the vector control cells and α-syn overexpression cells. More apoptotic cells were found in α-syn overexpression cells than in vector control cells(P<0.05). Conclusion α-Syn enhances H₂O₂-induced apoptosis of dopaminergic neuronal cells.

Key words: α-synuclein, hydrogen peroxide, apoptosis

CLC Number:

R 338

LI Ying;LIU Guang-wei;LI Xin;LI Yao-hua;YANG Hui;YU Shun. α-Synuclein promotes hydrogen peroxide-induced apoptosis of dopaminergic neuronal cells[J]. Journal of Capital Medical University, 2011, 32(6): 771-776.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2011.06.013

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2011/V32/I6/771

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α-Synuclein promotes hydrogen peroxide-induced apoptosis of dopaminergic neuronal cells

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