Expression of cytokines and immunoglobulin antibodies in the early asymptomatic and late symptomatic stage of allergic rhinitis mouse model

doi:10.3969/j.issn.1006-7795.2018.01.012

Journal of Capital Medical University ›› 2018, Vol. 39 ›› Issue (1): 63-68.doi: 10.3969/j.issn.1006-7795.2018.01.012

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Expression of cytokines and immunoglobulin antibodies in the early asymptomatic and late symptomatic stage of allergic rhinitis mouse model

Yang Jun¹, Wang Min¹, Zhang Luo^1,2

1. Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing Institute of Otorhinolaryngology, Key Laboratory of Otorhinolaryngology Head and Neck Surgery, Ministry of Education, Beijing Key Laboratory of Nasal Diseases, Beijing 100730, China;
2. Department of Allergy, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China

Received:2017-12-11 Online:2018-01-21 Published:2018-01-27
Supported by:
This study was supported by National Natural Science Foundation of China (81420108009, 81630023, 81570893),Changjiang Scholars and Innovative Research Team (IRT13082), Beijing Talent Project (2016), Natural Science Foundation of Beijing (7162042), Capital Special Projects for Scientific Research on Health Development(2014-4-1092),Beijing Health Bureau Program for High Level Talents (2009-2-007, 2011-3-043, 2014-3-018), Beijing Municipal Administration of Hospitals' Mission Plan (SML20150203), Beijing Scientific and Technological Overall Plan (Z171100000117002).

Abstract

Abstract: Objective To investigate the expression of interleukin-4 (IL-4), IL-17, IL-6 and immunoglobulin antibodies in early stage of allergic rhinitis (AR) response and their association with allergic symptoms.Methods At early stage (day 16) and late stage (day 30) of ovalbumin (OVA)-induced allergic rhinitis (AR) mouse model, nasal symptoms were measured; nasal mucosa was collected to detect the gene expression of interferon-γ(IFN-γ), IL-4, IL-17 and IL-6; serum was collected to detect the levels of OVA-specific IgE, IgG1, IgG2a and protein expression of IFN-γ, IL-4, IL-17 and IL-6.Results The number of nasal rubbings and sneezes was increased significantly in late stage compared to early stage and control, and had no significant difference between early stage and control. The level of serum OVA-specific IgE was significantly higher in early stage and late stage than that in control, but has no significant difference between early stage and late stage. Serum OVA-specific IgG1and IgG2a, gene expression of IL-17 and IL-6 in nasal mucosa, and serum IL-17 and IL-6 protein had similar expression patterns. These variables were significantly higher in early stage and late stage than those in control, and significantly higher in late stage than those in early stage. Gene expression of IL-4 in nasal mucosa and serum IL-4 protein levels in late stage was significantly higher than those in control.Conclusion IL-17, IL-6, and allergen specific IgG1 and IgG2a may be involved in the early stage of host against allergen response, and their levels may be associated with allergic symptoms.

Key words: allergic rhinitis, allergic symptoms, immunoglobulin antibodies, interleukin-17(IL-17), interleukin-6(IL-6)

CLC Number:

R765.21

Yang Jun, Wang Min, Zhang Luo. Expression of cytokines and immunoglobulin antibodies in the early asymptomatic and late symptomatic stage of allergic rhinitis mouse model[J]. Journal of Capital Medical University, 2018, 39(1): 63-68.

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Expression of cytokines and immunoglobulin antibodies in the early asymptomatic and late symptomatic stage of allergic rhinitis mouse model

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