Ethanol induces death of neuroblastoma cells by activating JNK and p38K pathways

doi:10.3969/j.issn.1006-7795.2012.06.022

Journal of Capital Medical University ›› 2012, Vol. 33 ›› Issue (6): 814-817.doi: 10.3969/j.issn.1006-7795.2012.06.022

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Ethanol induces death of neuroblastoma cells by activating JNK and p38K pathways

MOON Yong¹, KWON Yongil³

1. Department of Public Health Administration, Namseoul University, Cheonan 331-707, Korea;2. Department of Obstetrics and Gynecology, Kangdong Sacred Heart Hospital, Hally University, Seoul 150-030, Korea;3. Department of Neurobiology, Xuanwu Hospital, Capital Medical University, Beijing 100053, China

Received:2012-07-05 Revised:1900-01-01 Online:2012-12-21 Published:2012-12-21

Abstract

Abstract: Objective To study the mechanism underlying the ethanol-induced damage to SK-N-SH neuroblastoma cells. Methods MTT reduction assay was used to evaluate the cell death rate induced by ethanol. DNA fragmentation analysis, DAPI nuclear staining, and caspase-3 activity assay were applied to examine the apoptotic alterations. Western blotting analysis was used to observe the expressions of JNK and p38K. Results Ethanol induced dose-dependent cell death in SK-N-SH cells, which was mediated via apoptosis, as indicated by increased caspase-3 activity, nuclear disruption, and DNA fragmentation. The ethanol-induced apoptosis was partially mediated by the activated JNK and p38K pathways. Conclusion Ethanol mediates cell death of SK-N-SH neuroblastoma cells by activation of JNK and p38K pathways.

Key words: ethanol, apoptosis, neuroblastoma cells

CLC Number:

R338

MOON Yong;KWON Yongil. Ethanol induces death of neuroblastoma cells by activating JNK and p38K pathways[J]. Journal of Capital Medical University, 2012, 33(6): 814-817.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2012.06.022

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2012/V33/I6/814

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Ethanol induces death of neuroblastoma cells by activating JNK and p38K pathways

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