Abstract: To establish a simple,typical experimental animal model of acute necrotizing pancreatitis, rats were given 2.5 mg/g body weight of L-Arg intraperitoneal injection twice at an interval of 1h. Blood samples were collected for determination of serum amylase, and the pancreas were removed for the histological examination and the pancreatic wet/dry weight ratio delection. Result: The serum amylase of rats given L-Arg were significantly increased.The histological examination revealed that pancreas degeneration and necrosis,and the pancreatic wet/dry weight ratio were markedly increased. It shows that the administration of L-Arg overdoses seperately can induce a simple, reproductive experimental animal model of ANP.

Key words: pancreatitis, L-Arg, animal model