Abstract: All 48 screened sera were unknown etiological hepatitis. Four kinds of autoantibodies, including ANA,AMA SMA and anti-liver/kidney microsomal(LKM), were detected by indirect immuneofluorescence. TTV DNA were tested by nest PCR. Twenty-two of 48 patients(45.8%)were found with autoantibodies, 15(31.3%)with ANA, 5(10.4%)with AMA, 4(8.3%)with SMA, none with LKM. Seven of 48 patients(14.6%)were finally diagnosed as sufferering from autoimmune diseases: 3 had antoimmune hepatitis; 2 primary billiary cirrhosis and 2 primary Sjogren′s syndrome. M∶F=1∶6. Fourteen of 36(38.9%)were positive for TTV DNA. M∶F=2∶1. Three were also found to have autoantibodies in their sera. Autoimmune diseases and TTV infection prescented in Non-A, Non-G hepatitis. Severe damage in liver function could be seen in TTV patients, meanwhile autoimmune reaction was produced in some of them.

Key words: hepatitis, virus, autoimmune