Abstract: A hepatocellular carcinoma model of Wistar rat was constructed to study the expression of ICAM-1 in hepatocellular carcinoma and remote organs and to assess the growth, metastasis and invasion of cancer cell after the occlusion of hepatic vein. The change of expression of ICAM-1 in liver cancer, paracarcinoma tissue, nearby hepatic lobe and lung were measured by EnVision immunohistochemistry and the quantity of ICAM-1 in plasma by immunosurvey. The results showed that ICAM-1 in hepatocellular carcinoma tissue samples significantly downgraded after occulsion of hepatic vein compared with the control group(P<0.01). Positive samples of expression in paracarcinoma tissue(33.3%)and positive sample of expression in the left median lobe of the lever(39.6%)were fewer than the negative samples; while there were fewer positive samples than negative samples in hepatic left median lobe. However, we didn't find obviously discrepancy in contrast to the control group(P>0.05). The expression of ICAM-1 in the lung was higher than the control group(P<0.05). In plasma, the quantity of ICAM-1 extremely increased after the operation(P<0.01). Nevertheless, there wasn't a marked change found in the control group from pre-operation to post-operation. Therefore, we have the conclusion that the metastasis of malignant cell is different in different organs or tissues, and occlusion of hepatic vein accelerates cancer cell's transfer to remote part(lung)of body.

Key words: liver cancer, hepatocellular carcinoma, hepatic vein occlusion, ICAM-1, metastasis