Abstract: Objective To study the factors which affect the growth rate of the primary culture of the laryngeal squmous cell carcinoma. Methods Seventeen specimens of resected laryngeal squamous cell carcinoma tissues were obtained for primary cell culture and each of them was dissected in three pieces. Three pieces were dissociated by shearing, collagenase and physical methods, respectively. The results of these three methods and other factors were analyzed by χ² test. Results Cell growth rate were 64.7% in shearing group, 58.8% in collagenase group and 11.8% in physical group. The growth rate between the shearing group and collagenase group had no significant difference (P=1.000) while that of the physical group was much less than the other two groups (P=0.004, P=0.008). The tumor differentiation affected the growth rate. For well differentiated tumors the growth were 33.3% and for mildly/poorly differentiated ones the rate were 90.9%(P=0.028). In the study, there was no correlation between the TNM stage and the growth rate. Conclusion The growth rate is higher with tumors from patients with poor differentiated cells. The shearing method has high cell growth rate and less procedure, so it is the best method.

Key words: laryngeal neoplasms, carcinoma, squamous cell, cell culture, tissue dissociation