Abstract:

Objective To obsere influence of RGDS peptide on rat platelet aggregation rate and the ERK1/2 phosphorylation induced by different agonists in vivo and study the relationship between the change of ERK1/2 phosphorylation and the change of platelets aggregation with RGDS peptide. Methods Maximal aggregation rate(MAR) was measured with nephelometry; ERK1/2 phosphorylation was detected by Western blot. Results The MAR and phosphorylation of ERK1/2 were inhibited by RGDS peptide. Conclusion The phosphorylation of ERK1/2 was one of the cellular signal transduction mechanisms of the platelets aggregation; and the inhibition of ERK1/2 phosphorylation of may be one of the mechanisms that RGDS peptide antagonize thrombus.

Key words: ERK1/2, platelets aggregation, RGDS peptide, cellular signal transduction