Assessing stiffness of carotid artery via two-dimensional speckle-tracking in mice

doi:10.3969/j.issn.1006-7795.2018.06.016

Abstract

Abstract: Objective To explore a novel non-invasive method for the assessment of carotid artery stiffness by advanced speckle tracking algorithms by high-resolution ultrasound in mice. Methods Six 8-week-old C57B1/6J male mice and six 60-week-old Apolipoprotein E knockout mice (ApoE^-/-) were subjected to t ultrasound scan imaging through the Vevo 2100 with the MS-700 at a center frequency of 50MHz. Carotid intima-media thickness (IMT), mean diameter, mean area, area percent spread, diameter percent spread, distensibility, pulse wave velocity and global radial strain were quantified to evaluate the stiffness of the carotid artery wall in mice. Results Compared with C57 mice, ApoE^-/- mice had increased IMT[(0.05±0.01)mm vs (0.02±0.01)mm] but decreased area percent spread[(49.64±5.69)% vs (34.33±5.92)%], diameter percent spread[(25.21±3.04)% vs (17.33±3.14)%] and distensibility[(100.49±12.40)MPa^-1 vs (59.87±21.22)MPa^-1]. The pulse wave velocity was increased[(1.90±0.37)m/s vs (3.09±0.23)m/s], the global radial strain was reduced[(66.97±10.28)% vs (41.64±8.51)%] in ApoE^-/- mice. The difference was statistically significant (n=6, P<0.05). Conclusion Our study suggested that the two-dimensional speckle tracking technology was feasible for evaluating the stiffness of carotid artery wall in mice, thus providing a novel non-invasive method for the vessel stiffness assessment in rodents.

Key words: high-resolution ultrasonography, two-dimensional speckle-tracking, stiffness, noninvasive detection, mice

CLC Number:

R543.4

Xu Qing, Liu Lina, Song Yanting, Qu Aijuan. Assessing stiffness of carotid artery via two-dimensional speckle-tracking in mice[J]. Journal of Capital Medical University, 2018, 39(6): 883-888.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2018.06.016

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2018/V39/I6/883

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