Abstract:

Objective To investigate the effects on the gastrointestinal motility of ascites associated with experimental acute pancreatitis and on the electrogastrointestinal arrhythmia in rats and its mechanisms.Methods AP was produced with 5% sodium taurocholate by the method of retrograde pancreatic-injection. At 24 h after the induction of pancreatitis, the rats were sacrificed and the peritoneal exudates were aseptically collected followed by centrifugation at 4 ℃. The supernatants from the rats were combined which were designated as pancreatitis-associated ascitic fluid (PAAF). A total of 24 Wister rats were randomly divided into 4 groups: normal group, sham group, PAAF group and LNNA plus PAAF group. We completed directly A/D change and achieved the computer real-time recording by combining the traditional recording device with Maclab/4e date recording and analysis systems. Furthermore, the nNOS located in yenteric neurons was assayed by semi-quantitatived immunohistochemical methods in rat jejunum.Results The frequency and amplitude of the slow wave (SW) of the rat jejunum were inhibited when 5 mL PAAF was injected peritoneally after 24 h (PAAF group); while the percentage of dysrhythmias was increased( P <0.05),the contractive function of the intestinal smooth muscle were also inhibited more significantly than that of the sham group( P <0.05). The gray values in the yenteric neurons in rat jejunum of the PAAF group were increased obviously than that of the sham group( P < 0.05).Conclusion PAAF has effects on the electrogastr ointestinal arrhythmia in rats and the mechanisms may be related with the activation of nNOS in jejunum myenteric plexus during acute pancreatitis.

Key words: acute pancreatitis, ascitic fluid, gastrointestinal molitity, NOS