Cloning and Expression of Heparin-binding Haemagglutinin Adhesin of Mycobacterium Tuberculosis and Its Immungenicity

Journal of Capital Medical University ›› 2006, Vol. 27 ›› Issue (2): 162-165.

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Cloning and Expression of Heparin-binding Haemagglutinin Adhesin of Mycobacterium Tuberculosis and Its Immungenicity

Li Chuanyou, Zhang Xuxia, Zhang Jianyuan, Li Weimin, Tian Miao, Qiu Yunqing, Sun Zhaogang, Li Yan, Duanmu Hongjin

Department of Bacteriology and Immunology, Beijing TB & Thoracic Tumor Research Institute

Received:2006-02-28 Revised:1900-01-01 Online:2006-04-24 Published:2006-04-24

Abstract

Abstract: Objective To clone and express hbhA gene of M.tuberculosis,and observe production of anti-HBHA antibody and its immunogenicity from mouse serum.Methods 1) The hbhA coding gene of M.tuberculosis were amplified by PCR method.2) hbhA gene was cloned into E.coli for expression of specific protein.3) HBHA was injected into mouse and observed production of anti-HBHA antibody by ELISA method.Results 1) The hbhA gene of M.tuberculosis could be cloned into PET-32a(+) expression vector,and got a high expression.2) The recombinant HBHA increased antibody titer more than 1000000 times of immunized mouse compared with control group.Conclusion HBHA of M.tuberculosis has good immunogenicity.

Key words: Mycobacterium, tuberculosis, adhesin, expression, immunogenicity

CLC Number:

Q939.91
R521

Li Chuanyou;Zhang Xuxia;Zhang Jianyuan;Li Weimin;Tian Miao;Qiu Yunqing;Sun Zhaogang;Li Yan;Duanmu Hongjin. Cloning and Expression of Heparin-binding Haemagglutinin Adhesin of Mycobacterium Tuberculosis and Its Immungenicity[J]. Journal of Capital Medical University, 2006, 27(2): 162-165.

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Cloning and Expression of Heparin-binding Haemagglutinin Adhesin of Mycobacterium Tuberculosis and Its Immungenicity

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