Preparation and Identification of gp120 Transgenic Mice

Abstract

Abstract: Objective To construct HIV-1 B' subtype gp120 transgenic vector and prepare gp120 transgenic mice.Methods gp120 gene was amplified and cloned to pSEAP2-control vector according to reading frame,then GFAP promoter was inserted.The function fragment with promoter-gp120 gene-enhancer was digested and extracted to prepare transgenic mice.The gene integration and mRNA transcription was validated by PCR and RT-PCR.Results GFAP promoter controlled gp120 transgenic vector was successfully constructed and validated by gene sequencing,Western blotting analysis showed that the secretion and tissue specificity of gp120 was high enough;The existence of gp120 mRNA transcription in transgenic mice was confirmed by RT-PCR.Conclusion gp120 transgenic vector was successfully constructed and founder mice were produced.

Key words: human immunodeficiency virus, gp120, dementia, animal model

CLC Number:

Q789

ZHANG Hong-hai;SUN Yu;LIU Ya-li;ZHANG Tong;WU Hao;CHEN De-xi. Preparation and Identification of gp120 Transgenic Mice[J]. Journal of Capital Medical University, 2010, 31(6): 695-700.

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Preparation and Identification of gp120 Transgenic Mice

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