Alpha-synuclein Promotes Internalization of Surface N-methyl-D-aspartate Receptors in Dopaminergic Neuronal Cells

Journal of Capital Medical University ›› 2010, Vol. 31 ›› Issue (6): 737-741.

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Alpha-synuclein Promotes Internalization of Surface N-methyl-D-aspartate Receptors in Dopaminergic Neuronal Cells

CHENG Fu-rong¹, LI Xin¹, LI Yao-hua¹, WAND Yu-lan², ZHANG Yan-li¹, YU Shun¹

1. Department of Neurobiology, Beijing Institute of Geriatrics, Xuanwu Hospital, Capital Medical University, Key Laboratory for Neurodegenerative Diseases of Ministry of Education;2. Central Laboratory, Xuanwu Hospital, Capital Medical University

Received:2010-08-09 Revised:1900-01-01 Online:2010-12-24 Published:2010-12-24

Abstract

Abstract: Objective To investigate the effect of α-synuclein(α-Syn) on surface N-methyl-D-aspartate(NMDA) receptors.Methods Immunofluorescent labeling and western blotting analysis were used to measure the alterations of NMDA receptors and Rab5b proteins.Hypotonic K+ shock was used to inhibit the internalization process.Rab5b antisense oligonucleotides were applied to suppress Rab5b expression.The MES 23.5 dopaminergic cells were used as model cells to observe the effect of α-Syn on the surface NR1 expression.Results Treatment of MES23.5 cultures with 10 μmol/L of α-Syn triggered a significant decrease of the surface NR1 expression.This effect of of α-Syn was blocked by hypotonic K+ shock and suppression of Rab5b expression.Conclusion α-Syn promotes internalization of surface NMDA receptors by increasing Rab5b expression.

Key words: α-synuclein, MES23.5 dopaminergic cells, NMDA, internalization

CLC Number:

Q189

CHENG Fu-rong;LI Xin;LI Yao-hua;WAND Yu-lan;ZHANG Yan-li;YU Shun. Alpha-synuclein Promotes Internalization of Surface N-methyl-D-aspartate Receptors in Dopaminergic Neuronal Cells[J]. Journal of Capital Medical University, 2010, 31(6): 737-741.

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Alpha-synuclein Promotes Internalization of Surface N-methyl-D-aspartate Receptors in Dopaminergic Neuronal Cells

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