Effects of angiotensin-(1-7) on oxidative stress and adiponectin in adipocytes

doi:10.3969/j.issn.1006-7795.2014.01.011

Abstract

Abstract:

Objective Although reactive oxygen species (ROS) contribute to glucose intolerance induced by the renin-angiotensin system (RAS) is well documented, the role of the newly discovered pathway of RAS, angiotensin Ang-(1-7)/Mas axis, in this process remains unknown. Here, we examined the effect of Ang-(1-7) on oxidative stress and adiponectin in adipocytes. Methods We treated fully differentiated 3T3-L1 cells with exogenous Ang-(1-7) to clarify its effects on ROS production. Intracellular ROS was measured by flow cytometry using dihydroethidium (DHE), and nitroblue tetrazolium assay. The cells were cultured in serum-free medium for 2 hours and added with glucose oxidase 50 mU/mL or 100 mU/mL for 12 hours to establish the oxidative stress model. We used these models to detect the role of Ang-(1-7) in oxidative stress of adipocytes. ROS production was slightly but significantly decreased in adipocytes treated with Ang-(1-7). Additionally, Mas receptor antagonist D-Ala7-Ang-(1-7) (A779) reversed the effect of Ang-(1-7) on oxidative stress. Furthermore, we also found that oxidative stress induced by glucose oxidase suppressed expression of adiponectin, an insulin-sensitive protein. However, the suppression of oxidative stress by Ang-(1-7) restored adiponectin expression, while A779 antagonize these changes induced by Ang-(1-7). Conclusion Ang-(1-7) can protect against oxidative stress and increase adiponectin expression in adipocytes. Ang-(1-7) may be a novel target for the improvement of insulin sensitivity by preventing oxidative stress.

Key words: angiotensin-(1-7), A779, oxidative stress, adiponectin

CLC Number:

R587.1

Liu Chang, Cao Xi, Yang Fangyuan, Zhang Xuelian, Yuan Mingxia, Yang Jinkui. Effects of angiotensin-(1-7) on oxidative stress and adiponectin in adipocytes[J]. Journal of Capital Medical University, 2014, 35(1): 45-50.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2014.01.011

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2014/V35/I1/45

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