Expression of neuronal hemoglobin alleviates α-synuclein-induced apoptosis in MES23.5 cells

doi:10.3969/j.issn.1006-7795.2016.06.016

Abstract

Abstract: Objective To explore the effect of neuronal hemoglobin (nHb) on α-synuclein(α-syn)-induced cell apoptosis. Methods myc vector or nHb was transiently transfected into MES23.5 cells with or without addition of exogenous α-syn. As identification of gene expression by immunofluorescence, Western blotting was used to detect the expression of α-syn and nHb. JC-1 staining was used to detect the mitochondrial membrane potential. The cell injuries were observed by TUNEL staining and MTT assay. Results nHb gene overexpression in MES23.5 cells lead to formation the complexes with α-syn in cytosolic and mitochondrial fraction, and reduced free nHb levels. Meanwhile, nHb could reverse α-syn-induced reduction of mitochondrial membrane potential and cell apoptosis. Conclusion Increase of nHb could alleviate α-syn-induced cell injuries.

Key words: α-synuclein (α-syn), hemoglobin, mitochondria, apoptosis

CLC Number:

R338

Yang Weiwei, Li Xuran, Li Xin, Li Xuying, Yu Shun. Expression of neuronal hemoglobin alleviates α-synuclein-induced apoptosis in MES23.5 cells[J]. Journal of Capital Medical University, 2016, 37(6): 799-805.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2016.06.016

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2016/V37/I6/799

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