Chirality controls mitochondrial function of mesenchymal stem cells in three-dimensional microenvironment

doi:10.3969/j.issn.1006-7795.2022.03.018

Journal of Capital Medical University ›› 2022, Vol. 43 ›› Issue (3): 440-445.doi: 10.3969/j.issn.1006-7795.2022.03.018

• Basic Research • Previous Articles Next Articles

Chirality controls mitochondrial function of mesenchymal stem cells in three-dimensional microenvironment

He Ying¹ , Feng Chuanliang², Liu Jinying³, Zheng Huiming¹, Jiang Shengjie^1*

1. Department of Geriatric Dentistry,Peking University School and Hospital of Stomatology, Beijing 100034, China;
2. State Key Laboratory of Metal Matrix Composites School of Materials Science and Engineering, Shanghai Jiaotong University, Shanghai 200030, China;
3. Key Laboratory for Special Functional Materials of Ministry of Education School of Materials Science and Engineering, Henan University, Zhengzhou 450001, China

Received:2022-01-27 Online:2022-06-21 Published:2022-06-01
Contact: *E-mail:277062365@qq.com
Supported by:
National Natural Science Foundation of China(51833006, 82101072, 52003072).

Abstract

Abstract: Objective To analyze the chirality mediated mitochondrial function of stem cells and regulation of osteogenic differentiation. Methods Mesenchymal stem cells (MSCs) were cultured in vitro to monitor the mitochondrial membrane potential function of stem cells in vitro. In vivo, the expression of CXCL2 factor was observed in the early defect area after hydrogel implantation, and the difference between the related mitochondria and metabolic signaling pathways was analyzed by high throughput analysis in vitro. Results The proportion of mitotracker deep red immunoflourence staining decreased in the righ-handed group, which indicated that the mitochondrial membrane potential of stem cells was damaged in the right-handed group. In the rat cranial defects model, the expression of CXCL2 in the left-handed matrix repair area was up-regulated after early implantation. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis found that mitochondrial related genes and signaling pathways were significantly differentially between different chiral groups. A global microarray gene analysis showed that the expression of mitochondrion degradation related genes up-regulated in right-handed group. The results showed that left-handed matrix could up regulate the mitochondrial NADH dehydrogenase expression, while the mitochondrial function of mesenchymal stem cells in right-handed matrix was significantly damaged. Conclusion The chirallity of biological implant materials was able to regulate the function of stem cell mitochondria.

Key words: chirality, mitochondrion, mesenchymal stem cells

CLC Number:

Q731

He Ying , Feng Chuanliang, Liu Jinying, Zheng Huiming, Jiang Shengjie. Chirality controls mitochondrial function of mesenchymal stem cells in three-dimensional microenvironment[J]. Journal of Capital Medical University, 2022, 43(3): 440-445.

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References

[1] Engler A J, Sen S, Sweeney H L, et al. Matrix elasticity directs stem cell lineage specification[J]. Cell, 2006, 126(4): 677-689.
[2] Harada M, Karakawa S, Miyano H, et al. Simultaneous analysis of D,L-amino acids in human urine using a chirality-switchable biaryl axial tag and liquid chromatography electrospray ionization tandem mass spectrometry[J]. Symmetry, 2020, 12(6): 913.
[3] Wei Y, Jiang S J, Si M T, et al. Chirality controls mesenchymal stem cell lineage diversification through mechanoresponses[J]. Adv Mater, 2019, 31(16): e1900582.
[4] Jiang S J, Zeng Q, Zhao K, et al. Chirality bias tissue homeostasis by manipulating immunological response[J]. Adv Mater, 2022, 34(2): e2105136.
[5] 李园琦, 林海, 罗红蓉, 等. 线粒体自噬与骨髓间充质干细胞成软骨分化的关联[J]. 中国组织工程研究, 2020, 24(31): 4954-4960.
[6] Cossu G, Birchall M, Brown T, et al. Lancet commission: stem cells and regenerative medicine[J]. Lancet, 2018, 391(10123): 883-910.
[7] He P, Williams B A, Trout D, et al. The changing mouse embryo transcriptome at whole tissue and single-cell resolution[J]. Nature, 2020, 583(7818): 760-767.
[8] Killaars A R, Grim J C, Walker C J, et al. Extended exposure to stiff microenvironments leads to persistent chromatin remodeling in human mesenchymal stem cells[J]. Adv Sci, 2019, 6(3): 1801483.
[9] Suzuki M, Sujino T, Chiba S, et al. Host-microbe cross-talk governs amino acid chirality to regulate survival and differentiation of B cells[J]. Sci Adv, 2021, 7(10): eabd6480.
[10] Morganti C, Bonora M, Marchi S, et al. Citrate mediates crosstalk between mitochondria and the nucleus to promote human mesenchymal stem cell in vitro osteogenesis[J]. Cells, 2020, 9(4): 1034.
[11] 曹珈琪, 林文, 丁磊, 等. 线粒体DNA甲基化的研究进展[J]. 中国当代医药, 2020, 27(6): 17-21.
[12] Skok M. Mitochondrial nicotinic acetylcholine receptors: mechanisms of functioning and biological significance[J]. Int J Biochem Cell Biol, 2022, 143: 106138.
[13] Hayakawa K, Esposito E, Wang X H, et al. Transfer of mitochondria from astrocytes to neurons after stroke[J]. Nature, 2016, 535(7613): 551-555.
[14] Roger A J, Muñoz-Gómez S A, Kamikawa R. The origin and diversification of mitochondria[J]. Curr Biol, 2017, 27(21): R1177-R1192.
[15] Hijazi I, Wang E, Orozco M, et al. Peroxisomal support of mitochondrial respiratory efficiency promotes ER stress survival[J]. J Cell Sci, 2022, 135(1): jcs259254.

Chirality controls mitochondrial function of mesenchymal stem cells in three-dimensional microenvironment

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