Role of mitochondrial deacetylated protein SIRT3 on the cochlea of noise-induced hidden hearing loss

doi:10.3969/j.issn.1006-7795.2022.04.002

Abstract

Abstract: Objective To investigate the role of mitochondrial deacetylation protein sirtuin-3 (SIRT3) in noise-induced hidden hearing loss (NIHHL). Methods Injected SIRT3 inhibitor [3-(1H-1,2,3-triazol-4-yl) pyridine, 3-TYP] and untreated C57BL/6J mice were exposed to 100 dB white noise for 2 hours and hearing examination was performed within 24 h or fourteen days after noise exposure (NE). The changes of ribbon synapses (RS) of inner hair cells (IHCs) were observed by immunofluorescence staining. Via the frozen section, 4-hydroxynonenal (4HNE) staining was used to observe the accumulation of reactive oxygen species (ROS) in the cochlea. Results Compared with the control group, the NE group and 3-TYP group showed a temporary threshold shift, ROS accumulation and loss of ribbon synapses after NE. Compared with the NE group, the threshold of mice in 3-TYP group did not return to normal level 2 weeks after NE. We also found an obvious loss of ribbon synapses and the accumulation of ROS. Conclusion NE causes oxidative stress in the inner ear, and SIRT3 may protect auditory system function by resisting oxidative stress.

Key words: SIRT3, noise-induced hidden hearing loss, ribbon synapses, oxidative stress

CLC Number:

R764

Yang Zijing, Zhao Chunli, Liang Wenqi, Chen Zhongrui, Liu Ke, Gong Shusheng. Role of mitochondrial deacetylated protein SIRT3 on the cochlea of noise-induced hidden hearing loss[J]. Journal of Capital Medical University, 2022, 43(4): 521-526.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2022.04.002

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2022/V43/I4/521

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