Effect of baicalin on lipopolysaccharide-stimulated inflammatory response in human monocytes THP-1

doi:10.3969/j.issn.1006-7795.2022.06.012

Abstract

Abstract: Objective To observe the effect of baicalin on the lipopolysaccharide (LPS)-stimulated inflammatory response in human monocytes THP-1. Methods LPS was used to induce the inflammatory response of human monocyte THP-1, and the relative expression levels of interleukin-1β (IL-1β), IL-6, IL-8, granulocyte-macrophage colony-stimulating factor (GM-CSF) and tumor necrosis factor-α (TNF-α) were detected by real-time quantitative polymerase chain reaction (RT-qPCR) method to optimize the stimulation concentration of LPS.The cytotoxic effects of baicalein were also detected using the lactate dehydrogenase assay kit to optimize the bacailin concentration. The LPS-induced inflammation model of human monocyte THP-1 was pretreated with baicalin at different concentrations, and the inhibition effect of baicalin on the increasing expression of IL-1β, IL-6, IL-8, GM-CSF and TNF-α induced by LPS was examined by RT-qPCR. Results Compared to the control, the LPS treatment of human monocytes THP-1 at 1 μg/mL for 24 h could significantly increase the expression of IL-1β, IL-6, IL-8, GM-CSF and TNF-α (P<0.05).Treatment with baicalin at 10, 30 and 100 μmol/L on human monocytes THP-1 for 24 h and 48 h showed no significant cell cytotoxicity. The baicalein at 30 μmol/L and 100 μmol/L could significantly inhibit the LPS-induced increasing expression of IL-1β, IL-6, IL-8, GM-CSF and TNF-α in human monocytes THP-1. Conclusion The baicalin could significantly inhibit LPS-induced inflammatory response in human monocytes THP-1.

Key words: baicalin, lipopolysaccharide, human monocytes, inflammatory response, cytokine

CLC Number:

Wang Ping, Yang Jun, Wang Xiangdong, Zhang Luo. Effect of baicalin on lipopolysaccharide-stimulated inflammatory response in human monocytes THP-1[J]. Journal of Capital Medical University, 2022, 43(6): 893-898.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2022.06.012

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2022/V43/I6/893

References

[1] Fokkens W, Desrosiers M, Harvey R, et al. EPOS2020: development strategy and goals for the latest European Position Paper on Rhinosinusitis[J]. Rhinology, 2019, 57(3): 162－168.
[2] Shi J B, Fu Q L, Zhang H, et al. Epidemiology of chronic rhinosinusitis: results from a cross-sectional survey in seven Chinese cities[J]. Allergy, 2015, 70(5): 533－539.
[3] Tomassen P, Vandeplas G, Van Zele T, et al. Inflammatory endotypes of chronic rhinosinusitis based on cluster analysis of biomarkers[J]. J Allergy Clin Immunol, 2016, 137(5):1449－1456.
[4] Wang X D, Zhang N, Bo M Y, et al. Diversity of T_H cytokine profiles in patients with chronic rhinosinusitis: a multicenter study in Europe, Asia, and Oceania[J]. J Allergy Clin Immunol, 2016, 138(5): 1344－1353.
[5] Turner J H, Chandra R K, Li P, et al. Identification of clinically relevant chronic rhinosinusitis endotypes using cluster analysis of mucus cytokines[J]. J Allergy Clin Immunol, 2018, 141(5): 1895－1897.
[6] 张海波, 张宇玲, 赵丽敏, 等. 巨噬细胞在慢性鼻窦炎伴鼻息肉中的研究进展[J]. 国际耳鼻咽喉头颈外科杂志, 2020, 44(4): 221－224, 236.
[7] Hao D X, Li Y S, Shi J, et al. Baicalin alleviates chronic obstructive pulmonary disease through regulation of HSP72-mediated JNK pathway[J]. Mol Med, 2021, 27(1): 53.
[8] Zhou T, Zhang A J, Kuang G, et al. Baicalin inhibits the metastasis of highly aggressive breast cancer cells by reversing epithelial-to-mesenchymal transition by targeting β-catenin signaling[J]. Oncol Rep, 2017, 38(6): 3599－3607.
[9] Lu L W, Zhang M F, Wang X Y, et al. Baicalein enhances the antitumor efficacy of docetaxel on nonsmall cell lung cancer in a β-catenin-dependent manner[J]. Phytother Res, 2020, 34(1): 104－117.
[10] Zhao H, Li C D, Li L N, et al. Baicalin alleviates bleomycin-induced pulmonary fibrosis and fibroblast proliferation in rats via the PI3K/AKT signaling pathway[J]. Mol Med Rep, 2020, 21(6): 2321－2334.
[11] Liang W, Huang X B, Chen W Q. The effects of baicalin and baicalein on cerebral ischemia: a review[J]. Aging Dis, 2017, 8(6): 850－867.
[12] Lin C, Tsai F J, Hsu Y M, et al. Study of baicalin toward COVID-19 treatment: in silico target analysis and in vitro inhibitory effects on SARS-CoV-2 proteases[J]. Biomed Hub, 2021, 6(3): 122－137.
[13] Moghaddam E, Teoh B T, Sam S S, et al. Baicalin, a metabolite of baicalein with antiviral activity against dengue virus[J]. Sci Rep, 2014, 4: 5452.
[14] Bui T T, Piao C H, Song C H, et al. Baicalein, wogonin, and Scutellaria baicalensis ethanol extract alleviate ovalbumin-induced allergic airway inflammation and mast cell-mediated anaphylactic shock by regulation of Th1/Th2 imbalance and histamine release[J]. Anat Cell Biol, 2017, 50(2): 124－134.
[15] Yoshida K, Takabayashi T, Kaneko A, et al. Baicalin suppresses type 2 immunity through breaking off the interplay between mast cell and airway epithelial cell[J]. J Ethnopharmacol, 2021, 267: 113492.
[16] Li J, Lin X Y, Liu X, et al. Baicalin regulates Treg/Th17 cell imbalance by inhibiting autophagy in allergic rhinitis[J]. Mol Immunol, 2020, 125: 162－171.
[17] Chen S Z, Chen G R, Shu S, et al. Metabolomics analysis of baicalin on ovalbumin-sensitized allergic rhinitis rats[J]. R Soc Open Sci, 2019, 6(2): 181081.
[18] Yang M, Zhu X Y, Fu F D, et al. Baicalin ameliorates inflammatory response in a mouse model of rhinosinusitis via regulating the Treg/Th17 balance[J]. Ear Nose Throat J, 2022, 101(2_suppl): 8S－16S.
[19] Liu J Q, Wei Y, Luo Q L, et al. Baicalin attenuates inflammation in mice with OVA-induced asthma by inhibiting NF-κB and suppressing CCR7/CCL19/CCL21[J]. Int J Mol Med, 2016, 38(5): 1541－1548.