Adipose derived stem cells ameliorate experimental lymphedema

doi:10.3969/j.issn.1006-7795.2020.06.001

Abstract

Abstract: Objective To explore the feasibility of locally injected adipose derived stem cells (ADSCs) in treating lymphedema via reduction of CD4⁺T cells. Methods ADSCs isolated from subcutaneous adipose tissue of C57BL/6J mice were cultured in vitro to passage 3 to 5. Tail lymphedema was created on C57BL/6J mice by disconnecting both superficial and deep lymphatic vessels. Mice in total of 50 that received surgery were randomly assigned to two groups immediately after surgery. Animals in group 1 were injected subcutaneously with 15μL of PBS as control. Mice in group 2 were injected with 5×10⁵ nADSCs suspended in 15 μL of PBS. At every week after ADSCs injection, tail volumes of mouse tails were calculated. Tails were harvested at 3 weeks after injection. Subcutaneous adipose thickness, degree of fibrosis, lymphatic morphology and lymphatic reflux, were detected by Whole mount-BODIPY staining, Masson trichrome staining, immunofluorescence, and FITC-Dextran respectively. Results Transfer of ADSCs demonstrated an amelioration of lymphedema, which corresponded to a decrease in tail volume and nearly 40% decrease in adipose tissue thickness, as compared with controls. Using Masson trichrome staining, we found a 20% reduced collagen deposition after ADSCs transfer. Furthermore, the dramatic expansive lymphatic vessels were reduced by about 60% in area, while the total numbers were not changed. Transport function of lymphatic vessels was improved as evidenced by an increase in the amount of dye passing through the surgical incision within a limited time. Conclusion Local application of ADSCs alleviates lymphedema.

Key words: lymphedema, stem cells, adipose derived stem cells

CLC Number:

Zhou Chenxiao, Su Wanchun, Li Na, Liang Shuntao, Zhang Guoying, Ma Gang, Li Peilin, Cui Lei. Adipose derived stem cells ameliorate experimental lymphedema[J]. Journal of Capital Medical University, 2020, 41(6): 869-875.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2020.06.001

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2020/V41/I6/869

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