Abstract: Objective To establish human laryngeal squamous carcinoma cell line and define its biological charateristics.Methods Primary tumor tissue and the metastatic lymph node of laryngeal squamous carcinoma from a patient were used for culture with the regular small mass method.After 20 passages,their morphologies,growth curves,immunohistochemistry staining,cell cycles and colony-forming efficiency in soft agar were determined.Results Two human laryngeal squamous carcinoma cell lines were designated CCC-HLS and CCC-HLSLN respectively.CCC-HLS cells were main spindle,while CCC-HLSLN cells demonstrated various morphologies and some often had thin and long projection with branches in the end.Both of the cells were CK-positive. From the second day to the forth day,it was in the exponent growing stage for both cell lines and they began to die on the seventh day.The population doubling time of CCC-HLS cells was 33.9 h and that of CCC-HLSLN cells was 30 h.54.7% CCC-HLS cells were in G_O/G₁,5.2% in G₂/M and 40.1% in S,and 50.6% CCC-HLSLN cells in G_O/G₁,27.4% in G₂/M and 22.0% in S.The colony-forming rate of CCC-HLS cells was 1.23% and that of CCC-HLSLN cells was 2.58%.Conclusion We established two human laryngeal squamous carcinoma cell lines and studied their biological characteristics systematically.These cells can be used for basic research on laryngeal carcinoma.

Key words: laryngeal squamous carcinoma, human, CCC-HLS, CCC-HLSLN