Effect of Alpha-synuclein on N-methyl-D-aspartic Acid-induced Cytotoxicity

Journal of Capital Medical University ›› 2010, Vol. 31 ›› Issue (6): 726-731.

• 帕金森病的发病机制与生物标志物 • Previous Articles Next Articles

Effect of Alpha-synuclein on N-methyl-D-aspartic Acid-induced Cytotoxicity

LI Xin¹, CHENG Fu-rong¹, LI Yao-hua¹, WAND Yu-lan², ZHANG Yan-li¹, YU Shun¹

1. Key Laboratory for Neurodegenerative Diseases of Ministry of Education;Department of Neurobiology, Beijing Institute of Geriatrics, Xuanwu Hospital, Capital Medical University;2. Central Laboratory, Xuanwu Hospital, Capital Medical University

Received:2010-10-27 Revised:1900-01-01 Online:2010-12-24 Published:2010-12-24

Abstract

Abstract: Objective To investigate the effect of α-Synuclein(α-Syn) on N-methyl-D-aspartic acid(NMDA)-induced cytotoxicity.Methods NMDA cytotoxicity was produced on MES 23.5 dopaminergic cells.MTS cell viability assay,AO/PI fluorescent labeling of apoptotic cells,and western blot analysis of caspase-3 expression were used for assessment of the NMDA cytotoxicity.Confocal microscopy with Ca²⁺ probe was applied for monitoring the change of intracellular Ca²⁺ concentration.Results NMDA of 5 mmol/L triggered a significant increase of the cell death rate as revealed by decreased cell viability and increased apoptotic cells,which was blocked by a specific NMDA receptor antagonist MK801.In addition,NMDA induced significant increase in caspase-3 expression and intracellular Ca²⁺ concentration.Pretreatment of the cells with α-Syn attenuated the NMDA-induced cell death,[Ca²⁺]i up-regulation and caspase-3 increase.Conclusion α-Syn reduced NMDA-induced cytotoxicity through a mechanism of preventing caspase-3 activation and NMDA-induced Ca²⁺ influx.

Key words: α-Synuclein, MES23.5 dopaminergic cells, N-methyl-D-aspartic acid(NMDA), cytotoxicity

CLC Number:

Q189

LI Xin;CHENG Fu-rong;LI Yao-hua;WAND Yu-lan;ZHANG Yan-li;YU Shun. Effect of Alpha-synuclein on N-methyl-D-aspartic Acid-induced Cytotoxicity[J]. Journal of Capital Medical University, 2010, 31(6): 726-731.

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Effect of Alpha-synuclein on N-methyl-D-aspartic Acid-induced Cytotoxicity

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