Journal of Capital Medical University ›› 2011, Vol. 32 ›› Issue (1): 79-83.doi: 10.3969/j.issn.1006-7795.2011.01.016

Previous Articles     Next Articles

Effects of Icariin on α-synuclein Over-expression and Ubiquitinproteasome System in Gene-transfected PC12 Cells

LIU Ying1,2,3, ZHANG Lan1,2, LI Ya-li1,2, LI Lin1,2*   

  1. 1. Department of Pharmacology, Xuanwu Hospital, Capital Medical University; 2. Key Laboratory for Neurodegenerative Diseases, Ministry of Education; 3. Department of Pharmacy, Beijing Children’s Hospital, Capital Medical University
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-02-21 Published:2011-02-21
  • Contact: LI Lin

PDF

409

Abstract:

Objective To explore the effects of icariin, an effective component of Epimedium, on the over-expression and aggregation of α-synuclein(α-Syn) and other protein in vitro and mechanisms of the effects.
Methods Different concentrations of icariin were incubated with α-Syn gene-transfected PC12 cells for 24 h. The change in α-Syn mRNA content in the cells was determined by RT-PCR. The protein expression of α-Syn, UCH-L1 and Parkin was measured by Western blotting.
Results Incubation of icariin(40~80 μmol/L) with α-Syn gene-transfected PC12 cells for 24 h significantly decreased α-Syn mRNA content, inhibited α-Syn protein expression and aggregation, and enhanced the protein expression of UCH-L1 and Parkin in the model cells.
Conclusion Icariin inhibited the over-expression and aggregation of α-Syn in gene-tranfected PC12 cells in vitro, and the mechanisms may be related to the inhibition of synthesis and the enhancement of degradation of α-synuclein.

Key words: icariin, epimedium, &alpha, -Synuclein, ubiquitin-proteasome system, PC12 cell, in vitro

CLC Number: