Differences in cardiomyocytes' survival by anti-β1-adrenergic receptor autoantibodies of different concentrations

doi:10.3969/j.issn.1006-7795.2019.03.015

Abstract

Abstract: Objective To investigate the terminal effects on cardiomyocytes of β₁-adrenergic receptor autoantibodies (β₁-AA) at different concentrations for different time. Methods Cell survival was detected after 10^-10, 10^-9, 10^-8, 10^-7, 10^-6 mol/L β₁-AA treating neonatal rat cardiomyocytes (NRCMs) and H9c2 cells for 24, 36, and 48 hours, while mitochondrial function (ATP content and mitochondrial membrane potential) of H9c2 cells was detected after 48 hours.Results The cell survival of NRCMs and H9c2 decreased significantly by β₁-AA in high-concentrations (10^-7, 10^-6 mol/L) at any time and the survival of H9c2 proliferated slightly in low concentrations (10^-10, 10^-9, 10^-8mol/L) at any time. ATP content of H9c2 was decreased by β₁-AA in high concentration (10^-6 mol/L), while increased in medium and low concentrations (10^-10, 10^-9, 10^-8, 10^-7 mol/L). The mitochondrial membrane potential of H9c2 was decreased by β₁-AA in high concentrations (10^-6 mol/L), while increased in low concentrations. Conclusion β₁-AA damage cardiomyocytes in high concentration while it showed the positive effects in low concentrations (ie, promoting ATP production and cell survival). And all the effects had no relation to time. In conclusion, our study provides a new foundation for the differences of cardiac function among β₁-AA positive population.

Key words: β₁-AA, cardiomyocyte, mitochondria

CLC Number:

R541.6

Wang Zhaojia, Wu Ye, Liu Dan, Wang Meili, Wei Xin, Liu Huirong. Differences in cardiomyocytes' survival by anti-β₁-adrenergic receptor autoantibodies of different concentrations[J]. Journal of Capital Medical University, 2019, 40(3): 402-408.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2019.03.015

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2019/V40/I3/402

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Differences in cardiomyocytes' survival by anti-β₁-adrenergic receptor autoantibodies of different concentrations

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