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Research progress of effects and mechanisms of paeony and total glucosides of paeony in treatment of immune-associated dermatoses Yang Yang, Chen Ruixuan, Min Li, Ma Zhijie, Mei Xueling, Journal of Capital Medical University    2024, 45 (1): 1-7.   DOI: 10. 3969/ j. issn. 1006-7795. 2024. 01. 001 Abstract （434）      PDF（pc） （1315KB）（198）       Knowledge map    Save Pharmacological studies have shown that white paeony has anti-inflammatory,immunomodulatory,hepatoprotective,and analgesic effects. In clinical practice, paeony and total glucosides of paeony are often used to treat autoimmune diseases. As the anti-inflammatory, immune-modulating and anti-oxidizing mechanisms of paeony and total glucosides of paeony are gradually understood,their clinical applications in immune-related and inflammatory skin diseases have been continuously expanded. The mechanisms such as anti-oxidation play an important role in enhancing efficacy,attenuating toxicity,and reducing disease recurrence rates. This article reviews the research progresses on the effects and mechanisms of paeony and total glucosides of paeony in the treatment of immune-related and inflammatory skin diseases, to provide a basis for in-depth research. Reference | Related Articles | Metrics

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The application of artificial intelligence in otolaryngology head and neck surgery Zhao Jinming , Wang Xiangdong , Zhang Luo Journal of Capital Medical University    2024, 45 (2): 175-180.   DOI: 10.3969/j.issn.1006-7795.2024.02.001 Abstract （333）      PDF（pc） （484KB）（191）       Knowledge map    Save Artificial intelligence (AI) technology is increasingly widely used in the medical field. As an important branch of medicine, otolaryngology head and neck surgery has gradually applied AI technology to improve the efficiency and accuracy of diagnosis, treatment and disease management. This article reviews the historical development of AI, applications in the medical field, the wearable devices, and AI used in otolaryngology head and neck surgery, it will explore the challenges AI faces in medical application and prospects for future development. Reference | Related Articles | Metrics

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Correlation between visceral fat area and abdominal aortic calcification in hemodialysis patients based on bioelectrical impedance methods Wang Xiaoqi, Yuan Dan, Shao Feng, Zhou Jingjing, Yang Fan, Li Zhongxin Journal of Capital Medical University    2024, 45 (1): 127-132.   DOI: 10. 3969/ j. issn. 1006-7795. 2024. 01. 020 Abstract （132）      PDF（pc） （703KB）（182）       Knowledge map    Save Objective  To investigate the correlation between visceral fat area (VFA) and abdominal aortic calcification in patients with maintenance hemodialysis (MHD). Methods  Via cross-sectional survey,MHD patients in Beijing Luhe Hospital,Capital Medical University from April 2019 to May 2020 with complete data were selected and subjected to body composition analysis,excluding malnourished patients. The patients were divided into 4 groups according to the degree of calcium by Abdominal Aortic Calcification Score (AACs),and the clinical characteristics and VFA level were compared. Multivariate Logistic regression was used to analyze the risk factors of abdominal aortic calcification (AAC),and the predictive value of VFA level to abdominal aortic calcification was analyzed by receiver operating characteristic (ROC) curve.  Results  A total of 152 patients with MHD were included in this study and divided into non-AAC group (n=32),mild AAC group (n=45),moderate AAC group (n=54) and severe AAC group (n=21). There were statistically significant differences in serum phosphorus,intact parathyroid hormone (iPTH),high density lipoprotein-cholesterol (HDL-C),VFA and whether diabetes mellitus was combined among 4 groups (P < 0.05). Univariate correlation showed that AACs score of non-malnourished MHD patients was significantly positively correlated with diabetes,serum phosphorus,iPTH,and VFA level (r=0.188,0.238,0.223,0.392,P < 0.05),was negatively correlated with HDL-C level (r=-0.228,P < 0.05). Multivariate Logistic regression analysis showed that VFA and serum phosphorus were independent risk factors for AAC in MHD patients. ROC curve showed that the area under curve (AUC) of VFA in non-malnourished MHD patients for predicting AAC was 0.725 (P < 0.05),which had high predictive value. Conclusion  For non-malnourished MHD patients,elevated VFA is associated with the severity of AAC and is an independent risk factor for AAC,which may be a biological indicator for predicting the occurrence of AAC. Reference | Related Articles | Metrics

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Analysis of differentially expressed proteins and regulatory networks in cardiomyocytes after silencing CHAF1B based on label-free protein mass spectrometry Kang Yanhong, Gu Aiqin, Zhang Ying, Huang Shuai Journal of Capital Medical University    2024, 45 (2): 312-321.   DOI: 10.3969/j.issn.1006-7795.2024.02.020 Abstract （176）      PDF（pc） （6236KB）（152）       Knowledge map    Save Objective  To analyze differentially expressed proteins in cardiomyocytes after chromatin assembly factor 1 subunit B (CHAF1B) gene knockdown and predict the regulatory network, so as to provide reference for finding the potential therapeutic targets which can promote myocardial cell repair. Methods  Cell transfection and Western blotting methods were used to screen effective small interfering RNA (siRNA). Effective siRNA was used to knock down CHAF1B in human cardiac AC16 cells and then cell viability was detected by cell counting kit-8 method. The total protein was extracted, quantified, reduced, alkylated and then cleaved into peptides by trypsin. The peptides were identified by liquid chromatography with tandem mass spectrometry method. Differentially expressed proteins were identified by searching UniProt protein resource. Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment, and protein-protein interaction networks (PPI) analysis were conducted. Results  The survival of cardiomyocytes was significantly inhibited after CHAF1B gene knockdown by effective siRNA; the identification results of label-free protein quantitative mass spectrometry showed that there were 69 differentially expressed proteins, of which 50 proteins were significantly up-regulated (fold change≥2，P＜0.05) and 19 were significantly down regulated (fold change≤0.5，P＜0.05). GO analysis showed that these proteins mainly participated in biological processes such as macromolecular composite subunit matrix, cell component biogenesis and assembly, mainly distributed in the cytoplasm, vesicles and other regions, and played molecular functions such as protein binding. KEGG pathway enrichment and PPI analysis showed that the differentially expressed proteins participated in 10 signaling pathways such as proteasome, aminoacyl tRNA biosynthesis, endocytosis, pyrimidine metabolism, and amino acid biosynthesis, etc. The significantly up-regulated proteins such as proteasome subunit alpha type-2 and beta type-7 as well as 26S proteasome regulatory subunit 6B and 10B participated in the proteasome pathway; seryl-tRNA synthetase, glycine-tRNA synthetase, glutamine-tRNA synthetase and lysine-tRNA synthetase mediated aminoacyl tRNA biosynthesis. The significantly down regulated proteins, including actin-related protein 2/3 complex subunit 3 and heat shock 70 000 protein 1-like, participated in endocytosis; ribonucleoside-diphosphate reductase large subunit mediated pyrimidine metabolism. The real time quantitative polymerase chain reaction results confirmed that after transfection with CHAF1B siRNA, the mRNA levels of the gene ARPC3, which synthesized the skeletal related protein 2/3 complex subunit 3, and the key gene QARS1 for aminoacyl tRNA biosynthesis, were significantly reduced in cardiomyocytes. Conclusion  CHAF1B is a key protein for the survival of cardiomyocytes and participates in the regulation of various biological processes in cardiomyocytes. Referring to its regulatory network can help identify intervention steps that promote myocardial cell repair. Reference | Related Articles | Metrics

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Effect of the ribosome biogenesis factor BMS1 on proliferation of neuroblastoma cells Guo Jinxin, Jia Anna, Zhan Shijia, Zhang Yao, Zhang Xuan, Guo Yongli, Chang Yan Journal of Capital Medical University    2025, 46 (2): 296-305.   DOI: 10.3969/j.issn.1006-7795.2025.02.016 Abstract （121）      PDF（pc） （9341KB）（145）       Knowledge map    Save Objective  To elucidate the functional role and underlying mechanisms of the ribosome biogenesis factor BMS1 in neuroblastoma (NB) cellular proliferation. Methods  We utilized the R2 genomics analysis and visualization platform to analyze the correlation between BMS1 expression levels and clinical characteristics of NB children. The BMS1 mRNA level in three human neuroblastoma cells SK-N-BE(2), BE(2)-C, IMR-32 and two normal cells hTERT RPE-1, IMR-90 was detected by real-time quantitative polymerase chain reaction (RT-qPCR). Two distinct small interfering RNA (siRNA) sequences were used to target BMS1 mRNA in NB cells SK-N-BE(2) and BE(2)-C, with normal cells hTERT RPE-1 serving as controls. We used RT-qPCR to quantify the mRNA levels of BMS1 and two key neuroblastoma-associated molecules (MYCN and p53). After transfection with siRNA, cellular proliferation was detected by various experimental approaches: crystal violet staining, real-time cell analysis (RTCA), colony-forming unit assay and immunofluorescence. Results  By analyzing two independent neuroblastoma clinical cohorts (GSE85047/NRC-283 and Westermann-144 datasets), it was found that the BMS1 mRNA level in MYCN-amplified NB was significantly higher than that in MYCN-non-amplified NB (P<0.05). Furthermore, the overall survival rate of NB children in the BMS1 high-expression group was decreased (P<0.05). Consistent with these clinical observations, the BMS1 mRNA level in NB cells SK-N-BE(2), BE(2)-C and IMR-32 was significantly higher than that in normal cells hTERT RPE-1, IMR-90 (P<0.05).  The targeted transient knockdown of BMS1 in NB cell lines SK-N-BE(2) and BE(2)-C resulted in decreased intracellular MYCN mRNA expression levels (P<0.05), significantly reduced cell proliferation capacity and colony-forming ability (P<0.05). Immunofluorescence revealed that the expression of Ki-67, a proliferation marker, was decreased (P<0.05). At the molecular level, RT-qPCR showed that  the p53 mRNA level was significantly elevated in the BMS1-knockdown groups (siBMS1-1# and siBMS1-2#) compared with the control group (P<0.05). However, transient knockdown of BMS1 had no significant impact on the proliferative capacity of normal cells hTERT RPE-1. Conclusion  BMS1 expression was up-regulated in MYCN-amplified NB and negatively correlated with the prognosis of the NB children. Mechanistically, interfering with BMS1 expression may transcriptionally activate p53 in NB cells, thereby inhibiting their proliferative ability, while having minimal impact on normal cells growth kinetics. These findings suggest that BMS1 serves as an important proliferation driver in NB and is expected to be a promising therapeutic target for NB children, particularly MYCN-amplified pediatric patients. Reference | Related Articles | Metrics

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Progresses in the drug treatment of diabetic retinopathy Zhu Lin, Xu Yongsong, Feng Xiaotong, Zhao Dong, Ke Jing Journal of Capital Medical University    2024, 45 (3): 438-445.   DOI: 10.3969/j.issn.1006-7795.2024.03.010 Abstract （1072）      PDF（pc） （505KB）（142）       Knowledge map    Save Diabetic retinopathy (DR) is one of the common microvascular complications of diabetes and it is the main cause of blindness in adults. In recent years,  the treatment of DR has been enriched, and the clinical and basic research related to drug therapy has made great progress.  This article summarized the current agents used to treat DR, including  calcium dobesilate (CAD), pancreatic kininogenase (PK), fenofibrate, anti-vascular endothelial growth factor (anti-VEGF) drugs, glucagon-like peptide-1 receptor agonist (GLP-1RA), sodium-dependent glucose transporter 2 inhibitors (SGLT-2i), finerenone, antioxidants, natural products and melatonin. Reference | Related Articles | Metrics

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Relationship between chronic diseases and benign prostatic hyperplasia in middle-aged and older Chinese adults: A cross-sectional study based on CHARLS database Ming Jie, Jin Song, Liu Zhanliang, Wang Zongwei, Niu Yinong Journal of Capital Medical University    2025, 46 (2): 252-262.   DOI: 10.3969/j.issn.1006-7795.2025.02.011 Abstract （555）      PDF（pc） （5147KB）（139）       Knowledge map    Save Objective  To explore the correlation between chronic diseases and the risk of benign prostatic hyperplasia (BPH) in middle-aged and older Chinese man.Methods  Data from the 2013 China Health and Retirement Longitudinal Study (CHARLS) were used, including 4 509 male participants aged 45 years and older. Chronic diseases and BPH diagnoses were obtained through a questionnaire survey. Logistic regression analysis was performed to identify independent risk factors for BPH. Restricted cubic splines (RCS) were used to explore the nonlinear relationship between variables and BPH prevalence, while stratified analyses were conducted to assess the differential impact of chronic diseases on BPH prevalence in different subgroups.Results  Compared to patients without BPH, those with BPH had a significantly higher prevalence of comorbid chronic diseases, including hypertension, diabetes, chronic respiratory diseases, chronic heart disease, stroke, chronic kidney disease, chronic digestive diseases, arthritis or rheumatism, depression, and memory-related disorders. Multivariable Logistic regression analysis indicated that factors such as the 10-item Center for Epidemiological Studies Depression Scale (CESD-10) scores (OR = 1.043, 95% CI: 1.022－1.063, P < 0.001), chronic respiratory disease (OR = 1.518, 95% CI: 1.143－1.998, P = 0.003), chronic heart disease (OR = 1.515, 95% CI: 1.143－1.998, P = 0.003),  chronic kidney disease (OR = 2.384, 95% CI: 1.799－3.137, P < 0.001), and chronic digestive disease (OR=1.427， 95%CI：1.129-1.796,P=0.003) were independently associated with the occurrence of BPH. RCS analysis demonstrated no non-linear association between age, BMI, and CESD-10 scores and BPH. Stratified analysis revealed that the influence of these chronic conditions on BPH remained constantly stable across different subgroups.Conclusion  BPH is commonly comorbid with various chronic diseases. Future treatment strategies for BPH should consider the shared pathological mechanisms between BPH and these chronic conditions, with a focus on integrated interventions targeting common pathways. Reference | Related Articles | Metrics

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Analysis of infiltration of neutrophils and macrophages in tissues of eosinophilic gastroenteritis mice Xu Longwei, Wang Yadan, Zhou Minsi, Wu Jing Journal of Capital Medical University    2025, 46 (2): 306-313.   DOI: 10.3969/j.issn.1006-7795.2025.02.017 Abstract （101）      PDF（pc） （5404KB）（130）       Knowledge map    Save Objective  To study the infiltration of neutrophils and macrophages in the tissues of eosinophilic gastroenteritis mice. Methods  Thirty female C57BL/6 mice aged 6-8 weeks were selected to induce the model of eosinophilic gastroenteritis in wild-type mice. They were sensitized by intraperitoneal injection of  ovalbumin （OVA） protein and they were gastric stimulated with OVA. The successful modeling of eosinophilic gastroenteritis was verified by measuring the weight change of mice, detecting OVA specific immunoglobulin E (IgE) and Immunoglobulin G (IgG) levels in peripheral blood of mice by enzyme-linked immunosorbent assay （ELISA）, and detecting intestinal eosinophils count by flow cytometry. The proportion of neutrophils and monocytes in peripheral blood and the proportion of neutrophils and macrophages in spleen, mesenteric lymph nodes, small intestine and colon tissues were detected by flow cytometry. Results  The mice model of eosinophilic gastroenteritis was successfully induced by OVA abdominal sensitization and gavage stimulation. In mice with eosinophilic gastroenteritis, the infiltration of monocytes in peripheral blood, macrophages infiltration of spleen, mesenteric lymph nodes, small intestine and colon tissues were increased. And the neutrophil were increased infiltrated in peripheral blood, small intestine and colon tissues. Conclusion  Macrophage and neutrophil were increased infiltrated in peripheral blood, intestinal tissue of mice with eosinophilic gastroenteritis. Furthermore, the infiltration of macrophage was increased in lymphoid tissues, such as spleen and mesenteric lymph nodes of mice with eosinophilic gastroenteritis. Reference | Related Articles | Metrics

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Effect of CD38 expression of tumor-infiltrating immune cells on the prognosis of esophageal squamous cell carcinoma with lymph node metastasis Shi Feng, ZhaoYanjie, Gao Ying, Song Qingkun Journal of Capital Medical University    2025, 46 (2): 210-215.   DOI: 10.3969/j.issn.1006-7795.2025.02.006 Abstract （1378）      PDF（pc） （3878KB）（129）       Knowledge map    Save Objective  To investigate the prognostic effect of CD38 in patients with esophageal squamous cell carcinoma patients (ESCC). Methods  A retrospective cohort study with 142 ESCC patients was performed. The clinicopathological features and expression of CD38, CD4, CD8, Ki-67, programmed cell death ligand 1(PD-L1) and programmed cell death 1(PD-1) in tumor and immune cells were evaluated. Results  Among ESCC patients with lymph node metastasis, the expression of CD38 had a significant association with the overall survival (P=0.037). The median overall survival was 14 months and 38 months among patients with low and high expression of CD38, respectively. The crude and adjusted hazard ratio of high CD38 expression was 0.46 (95%CI： 0.21-0.98) and 0.32 (95%CI： 0.13-0.76) respectively. The expression of CD38 had a negative correlation with PD-L1 expressed in tumor cells. Conclusion  The high expression of CD38 was associated with a better survival for ESCC with lymph node metastasis.  Reference | Related Articles | Metrics

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A morphological description of the humerus based on statistical shape modeling Gao Weilu , Jia Zhengfeng , Yang Changsen , Li Jiantao , Su Xiuyun , Zhang Licheng Journal of Capital Medical University    DOI: 10.3969/j.issn.1006-7795.2025.05.001 Accepted: 21 September 2025

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Journal of Capital Medical University    2025, 46 (2): 0-0.   Abstract （86）      PDF（pc） （472KB）（114）       Knowledge map    Save Related Articles | Metrics

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A study on the differences of cognitive functions between individuals dominated by Wood Element and Metal Element among the Five Elements people Dai Zhiqing, Lin Jin, Duan Yuhang, Teng Fei, Lyu Hongpeng, Jia Hongxiao Journal of Capital Medical University    2024, 45 (3): 458-465.   DOI: 10.3969/j.issn.1006-7795.2024.03.013 Abstract （258）      PDF（pc） （1710KB）（111）       Knowledge map    Save Objective  To provide a basis for enriching the theory of the Five Elements and the psychological connotation of traditional Chinese medicine (TCM) by means of investigating the differences in the cognitive functions and processing processes between individuals dominated by the Wood Element and those dominated by the Metal Element according to the Five Elements theory in the TCM.Methods  According to the previous devised Quantitative Standard for Facial Phenotype Classification of TCM Five Elements People, typical individuals with a dominant Wood or Metal Element were recruited. The Repeatable Battery for the Assessment of Neuropsychological Status (RBANS) cognitive function assessment tool, as well as the Top-down and Bottom-up cue target task paradigm were employed to evaluate their cognitive functions and attentional processing differences. Independent sample t-tests and rank sum tests were applied to analyze the differences in cognitive functions between the two groups of participants. Results  This research included 32 participants with a dominant Wood Element and 40 participants with a dominant Metal Element. In RBANS cognitive function assessment tool, compared to the Wood Element group, the Metal Element group exhibited significantly higher scores in the visuospatial dimension and its subtask line orientation (P＜0.05). In the bottom-up cue target task paradigm, the Wood Element group displayed a statistically higher short reaction time difference. In contrast, the Metal Element group demonstrated a significant higher long reaction time difference (P<0.05). Conclusion  In the theory of TCM Five Elements, the Wood Element group relied more on bottom-up cognitive processing, while the Metal Element group performed better in processing visuo-spatial information and conserving cognitive processing resources. Reference | Related Articles | Metrics

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Research progress in the mechanism and prediction of suicide attempt in patients with depression Ren Yanping, Wu Han, Wang Wen, Jin Wenqing, Li Rena Journal of Capital Medical University    2024, 45 (1): 88-96.   DOI: 10. 3969/ j. issn. 1006-7795. 2024. 01. 015 Abstract （444）      PDF（pc） （556KB）（110）       Knowledge map    Save Suicide is the act of individuals intentionally or voluntarily taking various means to end their lives. The global annual suicide mortality rate is approximately 16/100 000,which means approximately 703 000 people die from suicide every year,making it the leading cause of death. Suicide often occurs in patients among which mental illness, among which the most common is depression. Studying the mechanism of suicidal behavior in depression can provide important data support for developing measares to prevent  from suicidal ideation and suicidal behavior. The relevant factors for suicidal behavior include social,psychological,and biological factors. The article reviewed the advances related to suicidal behavior in magnetic resonance imaging,neurocognitive research,machine learning,ecological momentary assessment and digital phenotype,in order to provide reference for the development of early identification indicators for suicide risk. Reference | Related Articles | Metrics

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Clinical features and genetic analysis of very early-onset inflammatory bowel disease caused by interleukin-10 receptor A gene mutation Li Yujia, Guan Dexiu, Guo Shu, Guo Jing, Xu Xiwei Journal of Capital Medical University    2024, 45 (1): 140-148.   DOI: 10. 3969/ j. issn. 1006-7795. 2024. 01. 022 Abstract （167）      PDF（pc） （876KB）（109）       Knowledge map    Save To summarize the clinical features and genetic characteristics of children with very early onset inflammatory bowel disease (VEO-IBD) caused by mutations in the interleukin 10 receptor A gene (IL10RA). Methods  Retrospective analysis was conducted on the children with chronic diarrhea hospitalized in the  Department of Gastroenterology Beijing Children's Hospital,Capital Medical University,from March 2007 to May 2019,who were diagnosed as very early onset inflammatory bowel disease (IBD). Among them,15 cases were diagnosed because of IL-10RA gene mutation,and 15 cases with VEO-IBD caused by non IL-10RA mutations in the control group. Clinical characteristics and gene reports were statistically analyzed. Results  Children with VEO-IBD caused by IL-10RA gene mutations,11 cases of Crohn's disease (CD) and 4 cases of ulcerative colitis (UC). The clinical symptoms  were mainly chronic diarrhea (15/15 cases,100.0%) and hematochezia (15/15 cases,100.0%),Extraintestinal manifestations were oral mucosal ulcers (6/15 cases,40.0%),skin erythema (5/15 cases,33.3%),and perianal manifestations were recto-perineal fistula in 5 cases (5/15,33.3%),4 cases of anal fistula (4/15,26.7%), 3 cases of anal fissure (3/15,20.0%),1 case of coexistence of recto-perineal fistula and skin tag (1/15,6.7%), and systemic manifestations of IL-10RA mutation group, malnutrition 13 cases （13/15 cases, 86.7%） perianal diseae 13 cases (13/15 cases, 86.7%). The control group had malnutrition 6 cases (6/15 cases, 40.0%) and perianal disease 5 cases (5/15  cases,33.3%).Compared with the IL-10RA mutation group，  the difference was statistically significant (P<0.05). Among 15 children with IL-10RA mutations,a total of 9 mutation sites were detected,with c.301c>T (p.R101W) and c.537G>A (P.T179T) being the most common mutation sites. The IL-10RA mutation led to an increase in inflammatory factors,leading to intestinal inflammatory response. Both prothrombin time and partial thromboplastin time were significantly prolonged.Conclusion  Children with very early-onset inflammatory bowel disease caused by IL-10RA gene mutations have an earlier age of onset. In addition to gastrointestinal symptoms,extraintestinal manifestations and perianal lesions are more common. The most common lesions under colonoscopy are multiple ulcers in the colon,followed by inflammatory polyps. The most prevalent mutation sites were c.301c>T (p.R101W) and c. 537G>A (P.T179T). The IL-10RA mutation led to an increase in inflammatory factors,leading to intestinal inflammatory response.  Reference | Related Articles | Metrics

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Severity classification of post-cardiotomy cardiogenic shock Wang Yiwen, Li Chenglong, Hou Xiaotong Journal of Capital Medical University    2024, 45 (2): 363-370.   DOI: 10.3969/j.issn.1006-7795.2024.02.026 Abstract （168）      PDF（pc） （1464KB）（109）       Knowledge map    Save The severity classification of post-cardiotomy cardiogenic shock is important for developing a rational treatment plan and predicting prognosis. The aim of this article is to explore the definition, etiology, pathophysiology, and general management of post-cardiotomy cardiogenic shock, to provide a review of traditional hemodynamic classification and shock management, and to discuss the current state of research on severity classification and shock management. Reference | Related Articles | Metrics

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Application of the win ratio method to clinical trials in cerebrovascular disease Tian Ruobing, Xu Qin, Zhou Quan, Tian Xue, Li Xinya, Zheng Manqi, Wang Anxin Journal of Capital Medical University    2025, 46 (2): 191-196.   DOI: 10.3969/j.issn.1006-7795.2025.02.003 Abstract （1300）      PDF（pc） （1777KB）（107）       Knowledge map    Save Objective  To introduce the concepts and principles of the win ratio method and to analyze it in the context of a case study of a clinical trial in cerebrovascular disease. Methods  Based on the study of clopidogrel with aspirin in high risk patients with acute non disabling cerebrovascular events 2, and key prognostic factors, the outcome events were defined sequentially as ① time to death within 90 d, ② time to recurrence of ischemic stroke within 90 d, ③ time to moderate-to-severe hemorrhage within 90 d. Using clopidogrel combined with aspirin as the reference group, the winning ratio (Rw) of ticagrelor combined with aspirin was analyzed by the win ratio method, and the 95% confidence interval (CI) of Rw was estimated by the Bootstrap method and compared with the hazard ratio (HR) calculated by the competing risk model. Results  When only fatal events were considered, the win ratio method suggested that the ticagrelor group was significantly better than the clopidogrel group, Rw=2.00 (95%CI:1.52-2.47), and after stepwise inclusion of  ischemic stroke and moderate-to-severe hemorrhage recurrence, the win ratio method yielded a value of 1.29(95% CI:1.25-1.57), and the HR value from Fine and Gray competing risk regression was 0.78 (95% CI:0.65-0.95), both of which indicated that the efficacy of the ticagrelor group was superior to that of the clopidogrel group. Conclusion  The win ratio method can be used to analyze clinical trials with composite endpoints after prioritizing multiple outcome variables, showing the advantages of win ratio and its promising application in cerebrovascular disease research. Reference | Related Articles | Metrics

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Journal of Capital Medical University    2024, 45 (2): 371-375.   DOI: 10.3969/j.issn.1006-7795.2024.02.027 Abstract （147）      PDF（pc） （5596KB）（106）       Knowledge map    Save Reference | Related Articles | Metrics

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Cumulative exposure of serum uric acid to serum creatinine ratio and the risk of carotid plaque： a prospective cohort study Jin Rui, Wang Jinqi, Xu Zongkai, Liu Yueruijing, Zhao Xiaoyu, Tao Lixin Journal of Capital Medical University    2024, 45 (2): 281-288.   DOI: 10.3969/j.issn.1006-7795.2024.02.016 Abstract （150）      PDF（pc） （1550KB）（101）       Knowledge map    Save Objective  To explore the association between cumulative exposure indicators of serum uric acid to serum creatinine ratio (SUA/Scr) and incident carotid plaque. Methods  This study included 9 228 individuals who met the inclusion and exclusion criteria from the Beijing Health Management Cohort. The cumulative exposure, cumulative exposure burden and high exposure time of SUA/Scr were calculated based on the values of SUA/Scr in three physical examinations in 2010 to 2011 (visit 1), 2012 to 2013 (visit 2), and 2014 to 2015 (visit 3). Carotid plaque was diagnosed by carotid ultrasound, and the follow-up was until December 31, 2021. Logistic regression model was used to analyze the association between SUA/Scr cumulative exposure indicators and carotid plaque occurrence, and sensitivity analysis was performed by excluding the participants with the usage of antihypertensive drugs, or glucose-lowering drugs, or lipid-lowering drugs. Results  The mean age of the study population was (48.89±10.22) years, and 5 846(63.35%) participants were males. The participants were divided into four groups according to the quartiles of SUA/Scr cumulative exposure, namely Q1-Q4 groups. After adjusting for covariates in models, compared with Q1 group, the ORs (95%CIs) of incident carotid plaque in Q2, Q3 and Q4 groups were 1.221 (95%CI：1.066-1.400), 1.255(95%CI： 1.095-1.439) and 1.478 (95%CI：1.287-1.697). Cumulative exposure burden greater than 0 (OR=1.223，95%CI：1.109-1.349) and high exposure time of 6 years (OR=1.332，95%CI：1.171-1.515) were both associated with increased risk of carotid plaque occurrence. Sensitivity analysis excluded participants with the usage of any antihypertensive drugs, glucose-lowering drugs, or lipid-lowering drugs. The results were similar to those in main analysis. Conclusion Cumulative exposure, cumulative exposure burden and high exposure time of SUA/Scr were associated with increased risk of incident carotid plaque. In the primary prevention of carotid plaque, we should focus not only on SUA/Scr value at a single point time, but also on long-term monitoring of SUA/Scr values. Reference | Related Articles | Metrics

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Construction and activity identification of anti-OX40/anti-EGFR bispecific antibody Jin Ruina, Bian Haibo, Zhang Xiaomin, Yang Fan, Wang Wanping Journal of Capital Medical University    2024, 45 (2): 296-301.   DOI: 10.3969/j.issn.1006-7795.2024.02.018 Abstract （168）      PDF（pc） （3711KB）（101）       Knowledge map    Save Objective  To construct a bispecific antibody targeting epidermal growth factor receptor（EGFR） and OX40 and evaluate the function for tumor-specific T cell activation. Methods  The gene of anti-OX40/anti-EGFR bispecific antibody was cloned into eukaryotic expression vector, and then the constructed vector were transfected to 293F cells for the bispecific antibody purification. The binding activity of anti-OX40/anti-EGFR bispecific antibody with the cells expressing target proteins  were detected by flow cytometry. To identify the activation of T cells mediated by anti-OX40/anti-EGFR bispecific antibody, the activation of NF-κB signal activation was evaluated by Jurkat-OX40-NF-κB-GFP reporter cells and the activation of primary T cells was detected by interleukin-2（IL-2） and interferon-γ（IFN-γ） secretion of peripheral blood mononuclear cell(PBMC). Results  Anti-OX40/anti-EGFR bispecific antibody was successfully constructed and purified, and its binding ability to HEK293 cells expressing OX40 and EGFR was verified. Jurkat-OX40-NF-κB-GFP reporter cells were activated by the bispecific antibody with the crosslinking of A549 cells. Further, the anti-OX40/anti-EGFR bispecific antibody promoted the secretion of IL-2 and IFN-γ of PBMC. Conclusion  Anti-OX40/anti-EGFR bispecific antibody was successfully constructed which could specifically recognize OX40 and EGFR and activate tumor specific T cells. Reference | Related Articles | Metrics

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Fingerprints of standardized decoction of radix Paeoniae Alba and identification of its chemical components Yu Xiaohong, Ma Zhijie, Mei Xueling Journal of Capital Medical University    2024, 45 (2): 289-295.   DOI: 10.3969/j.issn.1006-7795.2024.02.017 Abstract （182）      PDF（pc） （3005KB）（92）       Knowledge map    Save Objective  To establish the fingerprints of the standard decoction of radix Paeoniae Alba, and to identify the active ingredients of radix Paeoniae Alba, in order to provide theoretical basis for the quality control and clinical application of radix Paeoniae Alba. Methods  Ten batches of radix Paeoniae Alba were collected and the standard decoction of radix Paeoniae Alba was prepared; the fingerprints of the standard decoction of radix Paeoniae Alba were established by ultra performance liquid chromatography (UPLC）, and the fingerprints were analyzed by the 2012 version of the Fingerprint Similarity Evaluation System for Traditional Chinese Medicine; and the common peaks were identified by comparing with the control product, and by checking the mass spectral data with the literature. Results  The fingerprints of 10 batches of standard decoction of radix Paeoniae Alba were identified with 22 common peaks, and the peaks were well separated, with the similarity of each sample>0.9. Five common peaks were recognized, which were catechin, albiflora, paeoniflorin,  pyrethrin II, and benzoylpaeoniflorin, respectively. Conclusion  The established fingerprint method has good precision, reproducibility and stability, and can provide a basis for the quality evaluation of radix Paeoniae Alba. Reference | Related Articles | Metrics