Table of Content

15 January 1999, Volume 20 Issue 1

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论著

Distribution of Spinal pre-EPSP on the Lateral Dentrite of the Crucain Carp Mauthner Cell

Gao Na;Tong Xuehong;Zhang Shuhua;Li Xiaoyi;Yu Fen;Zhang Maoxian;Zhang Yingcai

1999, 20(1):  1-4. 

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Spatial distribution of spinal pre-EPSP(pre-EPSP)on the lateral dendrite was explored with multiple intracelluar recording technique in 8 Mauthner cells of the crucian carps. The results showed that pre-EPSPwith maximal amplitude was recorded at the soma, from where its amplitude decayed progressively along the lateral dendrite. The style of decay was very comparable to that of antidromic action potential. It is suggested that: ① The synapses mediating pre-EPSP are located densely at the soma; ② As the antidromic action potential, the pre-EPSP propagates along the lateral dendrite simply in the manner of electrotonic spread. It means that no synapses mediating pre-EPSP impinge on the lateral dendrite.

Use of Monoclonal Antibody Norcantharidin Conjugate in Experimental Targeting Therapy of Tumor

Liang Fuyou;Zhang Shenghua;Pan Ying;Xu Linna;Wang Weigang

1999, 20(1):  5-8. 

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Arat monoclonal antibody was linked to norcantharidin (NCTD), an antitumor drug currently in clinical use McAb 3A5. The 3A5-NCTD conjugate retained complete immunoreactivity of McAb 3A5 to human hepatoma cancer BEL-7402 cells. The IC₅₀ values of 3A5-NCTD and free NCTD were 2.3 mg/Land 4.2 mg/L, respectively. The 3A5-NCTD showed higher cytotoxicity than free NCTD. Hepatoma H22 ascites was transplanted into the peritoneal of mice. 3A5-NCTD or NCTD were injected into the cavity. The ILS(%) values were 168% and 42%. Tumor fragments of human hepatoma BEL-7402 were transplanted into nude mice. Then 3A5 NCTD or NCTD was injected iv, or pt(peritumorally). The inhibition rates on the growth of hepatoma BEL-7402 xenografts were 62% and 15% for iv; 78% and 47% for pt.Results indicate that 3A5-NCTD is highly effective against targeted human cancer xenograft and mouse tumor.

Based on Deformable Surface Model Morphology Extraction of Cortical Surface in MR Images

Luo Shuqian;Yan Hua

1999, 20(1):  9-13. 

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As a new method to map the outer cortex of the human brain, a deformable surface model is described in this paper. Obtaining the shape of the brain is an important step for shape visualization, morphological analysis and registration of brain images.Using the deformable surface algorithm on three dimensional MRimages a parametererization of the central layer of the outer cortex can be obtained. This algorithm combines robustness to noise with flexibility to represent a broad class of shapes.

The Activities of Nitric Oxide Synthase in the Neurons of the Nigro striatal System in Rat Brain

Lü Heyi;Xu Qunyuan;Zhao Peng;Zhang Jinlu;Xu Jianlan

1999, 20(1):  14-16. 

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The activites of nitric oxide synthase(NOS) in cell bodies and terminals of neurons in the nigro striatal system were detected by using a histochemical method of nicotinamide adenosine dinucleotide phosphate diaphorase (NADPH-d) reaction in brain sections of the SD rat. The number of NOS positive neurons and density of the NOS positive terminals were counted under a DMRP biomicroscope. It was shown that the higher density of labled terminals and more labled neurons were located in the ventrolateral part of the striatum, while the lower density of terminals and less neurons in the dorsomedial part of the striatum. There were no terminals labled in the globus pallidus, substantial negra and thalamus. The results showed that the distributions of NOS positive neurons may have certain functional significance for motor regulation and certain parts of NOS positive terminals may be projected from the cerebral cortex.

Effect of Water Extract of Cordyceps sinensis and Paecilomyces sinensis on Immune Function in Mice

Zhang Chuankai;Yuan Shengrong;Liu Jinxue

1999, 20(1):  17-20. 

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Effect of water extract of Cordyceps sinensis (CS) and Paecilomyces sinensis (PS) on nonspecific and humoral immue function in mice was investigated. Charcoal particles clearance rate assay and peritoneal macrophages (PMφ) phagocytosis of neutral red were measured by colorimetric method as well as quantitative hemolysis spectrometric (QHS) assay. The results demons trated that both CS and PS 1.0, 2.0, 4.0 g/(kg·d)×3 d, ig dose dependently intensified charcoal particle clearance rate in mice (P<0.01) and significantly reversed cyclophosphamide (Cy) reduced clearance rate at the dose of 2.0, 4.0 g/(kg·d)×3 d, ig respectively (P<0.01). In vitro, both CSand PS (0.25～8.0 g/L) significantly enhanced PMφ to phagocytize neutral red in a concentration dependent manner (P<0.01), both CSand PS 4.0 g/(kg·d)×7 d, ig significantly enhanced antibody formation cell (AFC) and serum hemolysin IgM level in normal mice (P<0.01), makedly reversed Cy-depressed humoral immunity (P< 0.01). In conclusion, both CS and PS markedly strengthened nonspecific and humoral immune function in mice.

Beneficial Effects of Ginkgo Bilboa Injection on Experimental Focal Cerebral Ischemia in Rats

Ma Yi;Hei Ailian;Jin Youyu;Hu Xiaomin Department of Pharmacology;Capital University of Medical Sciences

1999, 20(1):  21-23. 

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Ginkgo bilboa injection, is extracted from ginkgo leaf. The protective effects of ginkgo bilboa extract (GBE) on focal cerebral ischemic injury were studied. Focal cerebral ischemia was produced by permanent occlusion of the proximal portion of the right middle cerebral artery (MCA) with the technique of Tamura. The neurologic status of each rat was carefully evaluated at 8 and 24 h after surgery. The cerebral infarction and water content were measured. The histological changes of brain tissue after MCAOin rats were also studied. Treatment with GBE 20 or 40 mg/kg iv was shown to significantly reduce the cerebral infarction and water content, attenuate neurological deficits. The degree of inhibition of cerebral infarction were 24.4% (P<0.05) and 34.7% (P<0.01), respectively. In pathological examination, the extent of tissue ischemia in the group of treatment with GBE 40 mg/kg was much less than that of control. These results indicate that GBE protects rats from ischemic neurological damage.

Morphologic Observation and Pathogenesis Discussion on Acute Haemorrhagic Necrotic Pancreatitis

Chen Ruifen;Song Aili;Liu Guozhen;Cao Wenjun;Yuan Biao;Chen Fulai

1999, 20(1):  24-27. 

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The rats were divided into three groups: Normal control group, acute pancreatitis model group and treated group by sodium fulvate(SF). In pancreas tail microcirculation was measured and pathologic sections were examined. It was found that SFhad beneficial effects on acute haemorrhagic necrotic pancreatitis. The results indicated that microcirculatory impairment may play an important role in pancreatitis pathogenesis. The theoretical basis was provided for clinical treatment.

Mechanism of Pulmonary Vascular Structure Remodeling Induced by Chronic Hypoxia

Liu Fan;Chen Ruifen;Liu Guozhen;Song Aili

1999, 20(1):  28-31. 

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Rat was used as the hypoxia model by being kept in the normobaric hypoxia chamber. The lung sections were stained with routine HE, VG and immunohistochemical methods. Some specimens were examined by electromicroscope. Results showed that the remodeling of the pulmonary vascular structure under chronic hypoxia included: ① significant proliferation of the adventitial collagen fiber in pulmonary arteries. ②hypertrophy of the medial coat of peripheral muscular arterioles and ③ muscularization of the nonvascular arterioles in the alveolar septa.

论著

Relation between Circadin Rhythm of Blood Pressure and Heart Rate Variability in Essential Hypertension

Du Fenghe;Xu Xiuying

1999, 20(1):  32-34. 

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To investigate the relationship between the circadin rhythm of blood pressure and the heart rate variability in essential hypertension, 65 cases of hypertensive disease were studied by means of mearsu ring the 24 h ambulatory blood pressure and the heart rate variability. The results showed that the patients with hypertension had lower SDNN and SDANNI as compared with the normal controls (n= 38, P <0.05) and these changes had more significance (SDNN, SDANNI and SDNNI P<0.05) in the patients without circadin changes of blood pressure than those with such changes. These results show that cardiac autonomotropic function was impaired more in the former than in the later patients.

A Efection of Early First Defibrillation Time on ROSC

Lu Junling;Wang Peiyan

1999, 20(1):  35-36. 

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To observe the effect of beginning defibrillation on ROSC the twenty six case records including 29 attacks of ventricular fibrillation were analyzed, and their differences were checked using χ² test method. The results showed that there existed significant differences on ROSC rate between the cases whose beginning defibrillation time was 3 min or shorter and the cases whose beginning defibrillation time was longer than 3 min(P<0.05).

Clinical Application of PCR Assay for Detection of Neisseria gonorrhoeae

Zhang Jingsheng;Xiao Bai;Tan Shuzhen;Yan Jingli;Cai Youling;Liu Jingzhong

1999, 20(1):  37-39. 

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Inder to evaluate the clinical application of PCR assay for the detection of Neisseria gonorrhoeae. Genitourinary specimens were obtained from NG patients and Neisseria gonorrhoeae was detected with a number of tests including Gram's stain of smears, culture and PCRassay. The sensitivity and specificity of the NG-PCR assay were evaluated with 8 bacterial strains. The NG-PCR assay had a detection limit of 3 copies of NG genomic DNA. In the specificity of the PCRassay, the standard Neisseria gonorrheae was positive, but no PCR product could be visualized in 7 nongonorrhoeae strains. PCRresults of 47 NG patients were compared with those obtained by Gram's smear and culture. The positive rates for smear, culture and PCRwere 76.6%, 52.2% and 95.9%, respectively. Of 82 normal controls, 1 specimen was PCR positive. The false positive rate of the PCRassay was about 1.2%. The PCR assay is a satisfactory diagnostic tool for the detection of Neisseria gonorrhoeae in clinical specimens.

The Rule of Magnetic Resonance Imaging in the Examination of Giant Cell Tumor of Limb Bone

Zhou Hai;Cao Yali;Xu Bing;Yang Rong;Xu Xiaotong

1999, 20(1):  40-42. 

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Eight cases of bone tumor were examined by MRI, CT and plain film. 5 cases MRI showed low signal intensity on T₁-WI and high signal intensity on T₂-WI. 3 cases of MRI showed mixed signal intensity on T₁ and T₂-WI. CTand plain film both showed cortical thinning and destruction. These results shown that MRI was superior to CT and plain film in demonstrating areas of tissue inhomogeneity within the tumor as well as soft tissue extension. CT was superior in demonstrating cortical destraction and subtal reation.

论著

A Etiological Analysis of Low Back Pain in 439 Outpatients

Liu Chongjing;Li Zhifang;Qu Tiebing

1999, 20(1):  43-44. 

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The causes of low back pain in 437 outpatients were analyzed. Result showed that most outpatients were old people and women. Patients with chronic lumbago constituted the majority. Main cause were chronic strain and degeneration of the lumbar tissue. Cause of lumbago is very complicated. It relates to loin tissue, patient's age and sex, work character enviromment, climate etc.

An Experimental Study on the Changes of the Estrogen Distribution in the Condylar Cartilages after Functional Mandibular Protrusion

Bai Yuxing;Luo Songjiao

1999, 20(1):  45-48. 

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Simulated functional maxillary inclined planes were used for experimental rats to guide mandibles forwards. The immunohistochemical ABC method was performed to analyze the distribution of estrodial in the mandibular condylar cartilages. The results showed that estrodial was distributed mainly in the germinal layers of the cartilages with more distribution during their actively proliferative period. Estrodial was located mainly in the nuclei of the chondrocytes. More distribution of estrodial was viewed after functional mandibular protrusion, accompanied with much E 2 positive staining. It is suggested that estrodials play an important role in promoting the growth and functional adaptive remodeling in the condylar cartilages.