Comparison of Apoptotic Effect between BT325 Human Glioblastoma and U251 Glioblastoma Inducted by FasL Gene Transferred NIH3T3 Fibroblast

Journal of Capital Medical University ›› 2006, Vol. 27 ›› Issue (5): 600-603.

• 基础研究 • Previous Articles Next Articles

Comparison of Apoptotic Effect between BT325 Human Glioblastoma and U251 Glioblastoma Inducted by FasL Gene Transferred NIH3T3 Fibroblast

Li Junjie, Zhao Jizong

Department of Neurosurgery, Beijing Tiantan Hospital, Capital University of Medical Sciences

Received:2006-04-19 Revised:1900-01-01 Online:2006-10-24 Published:2006-10-24

Abstract

Abstract: Objective To compare the effects of FasL gene on the induction of apoptosis between two kinds of human glioma cells in vitro.Methods FasL gene was introduced into NIH3T₃ fibroblast cell by liposome reagents.FasL expressed by the NIH3T₃/FasL cells was investigated with immunohistochemical staining and FasL mRNA was explored with RT-PCR.The ability of NIH3T₃/FasL cells to induce apoptosis of BT₃25 and U251 glioblastoma cocultured was assayed with Hochest 33342 fluorescent staining and TUNEL method respectively.Results FasL was expressed effectively in NIH3T₃/FasL cells,and compared with the control group,the experimental group had significant effect on the induction of apoptosis of BT₃25 and U251 cells((P<0.05)),the apoptosis of BT₃25 glioblastoma induced by NIH3T₃/FasL was more significant than that of U251 glioblastoma((P<0.05)).Conclusion FasL gene transferred NIH3T₃ fibroblast has greater apoptosis induction effect on BT₃25 glioblastoma than on U251 glioblastoma.

Key words: glioma, FasL, apoptosis

CLC Number:

R739.41

Li Junjie;Zhao Jizong. Comparison of Apoptotic Effect between BT325 Human Glioblastoma and U251 Glioblastoma Inducted by FasL Gene Transferred NIH3T3 Fibroblast[J]. Journal of Capital Medical University, 2006, 27(5): 600-603.

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Comparison of Apoptotic Effect between BT325 Human Glioblastoma and U251 Glioblastoma Inducted by FasL Gene Transferred NIH3T3 Fibroblast

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