Table of Content

15 July 2001, Volume 22 Issue 3

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论著

Effect of Nitric Oxide on the Cotical Neurons Apoptosis Induced by Hypoxia/ Reoxygenation and EGB Protection

Ji Fengqing;Sun Haimei;Yue Xu;Guo Yanru;Du Xiaoyu;Guo Chongjie;Zhao Tiande

2001, 22(3):  193-198. 

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To explore the effect of extract ginkgo biloba(EGB)on the dynamic expression of nitric oxide(NO)in the neuron apoptosis following hypoxia/reoxygenation(H/R), cerebral neurons of fetal Wistar rats were primarily isolated and cultured. Apoptosis of cultured cerebral cortical neurons following H/R was detected with Tunel and Flow cytometry method. The dynamic changes of NO metabolite nitrite(NO₂^-)in the supernatent solution of cultured neurons were measured by fluorospectrophotometry. No content in the apoptosis of cerebral neurons of fetal Wistar rats increased at H₂R₀ and H₁₈R₁₈ respectively to the normal control group. EGB showed an inhibition function to the increase of NO. AG inhibited the increase of NO at H₈R₁₈ , but showed no effect on the NO in H₂R₀ group. NO plays a role in the apoptosis of neurons following H/R, and EGB has a protective function on the apoptosis . The probable mechanism of EGB protection is its inhibition of NO.

Changes of SP and VIP in Ileum of Experimental Spleen Deficiency Rats

Guo Hua;Qu Reiyao;Chang Yanbin;Meng Yang;Li Lisheng;Wang Wei;Zeng Wenhong;Qu Bolin

2001, 22(3):  199-201. 

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Substance P(SP)and vasoactive intestinal peptide(VIP)levels in all layers of initial part of ileum in rats were measured by radioimmunoassays(RIA)in order to explore the mechanism of spleen deficiency(SD).SP levels in ileum of experimental SD rats were significantly higher than that of the control groups(P<0.005)while the VIP levels in ileum of SD rats were significantly lower than the control groups(P<0.05). In spontaneous recovery cases, SP levels recovered significantly(compared with the SD rats, P<0.001)and did not drop down to normal until the VIP levels recovered significantly. After the SD rats were treated with Chinese herbs(Sijunzi Tang), SP was improved(compared with the SD cases, P<0.001)and returned to normal(compared with the controls, P>0.10). So the results showed that changes of SP and VIP levels in initial part of ileum of SD rats may be closely related to the gastrointestinal motility disorders presented in SD and the Chinese herbs(Sijunzi Tang)currently used has a partially therapeutic effect.

The Effect of APP17-mer Peptide on the Neurotoxicity of Aβ

Wang Rong;Zhang Jingyan;Ji Zhijuan;Xu Yanling;Sheng Shuli

2001, 22(3):  202-205. 

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The present work aims at adducing further proof of the neurotrophic effect of β amyloid(Aβ)precursor protein(APP)319～335(APP17 mer peptide), through studying the effect of APP17 mer peptide on the neurotoxicity of Aβ, and thereby providing scientific evidence for the pathogenesis of AD and its treatment. APP17 mer peptide and Aβ_25-35 were synthesized by solid phase method and purified by HPLC. Human neuroblastoma cells SY5Y were segregated into 3 groups: normal control group, Aβ_25-35(10μmol/L)damaged group, and Aβ_25-35(10μmol/L)+APP17 mer peptide(10μmol/L)for neuroprotection group. Cell count, MTT metabolic rate, LDH leakage rate, axonal length, area of cell body, NT 3 immunohistorychemical staining and concentration of cytosolic calcium(Ca²⁺)were used as indicators. Compared with normal control group, Aβ_25-35 damaged group showed reduced cell count and MTT metabolic rate, increased LDH leakage rate, diminished axonal length and area of cell body, reduced NT 3 expression, increased concentration of cytosolic calcium(Ca²⁺), but the addition of APP17 mer peptide normalized the foregoing changes. APP17 mer peptide is neurotrophic. It can diminished neurotoxicity of Aβ.

Pretreatment with Puerarin Against Astrocyte Swelling in Culture and Brain Edema in Rats

Yuan Fang;Wang Zhongcheng;Wang Tianyou;Xu Lixin;Zhang Yazhuo

2001, 22(3):  206-208. 

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The effects of puerarin(pue), an effective component of a Chinese herb, against astrocyte swelling in cultures and brain edema intact rats were studied. Astrocyte swelling in culture was induced with trans-1-aminocyclopentane-1, 3-dicarboxylic acid(tACPD), an agonist of metabotropic glutamate receptors. tACPD(1mmol/L)alone or with pue(1mmol/L)were added in culture at 37 ℃ for 1 h, then the intracellular water space(IWS)was measured by ³H-3-O-methyl-D-glucose. For in vivo study, brain edema was induced with tACPD(500nmol)in 2 μL injected stereotactically into right caudatum of the rat. Pue(50mg/kg)was administered intraperitoneally on the 1st and 2nd day, and intravenously on the 3rd day, then followed by intracaudatal injection of tACPD. Sixh after tACPD injection brain water content and Na⁺, K⁺, Ca²⁺ contents were determined. Histological study was performed with electron microscope(EM). It was showed that pue blocked the increase of IWS induced by tACPD in astrocyte culture and reduced the increases of brain water and Na⁺ contents induced by tACPD in vivo. Furthermore, astrocyte swelling and the constriction of capillary lumen induced by the pressure of swelling endfeet of astrocytes, which were observed in tACPD treated rats under EM, were attenuated by pue treatment. The results indicats that pretreatment with pue can prevent astrocyte swelling and brain edema induced by tACPD, which may be beneficial to brain microcirculation during hypoxia and ischemia.

Effect of Thyrotropin-releasing Hormone Analogue, YM14673, on c-fos Protein Expression in Ischemic Neuron

Zhai Jing;Zhang Jiajin;Zhang Hui

2001, 22(3):  209-212. 

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To study the effect of thyrotropin releasing hormone analogue, YM14673, on c fos protein expression in rat hippocampus following cerebral ischemia, cerebral ischemia was produced by occlusion of middle cerebral artery(MCA)and bilateral common carotid arteries(CCAs)with small aneurysmal clips for 1 h in wistar rats. Cerebral coronal section(10 μm)was routinely stained with hematoxylin eosin(HE)and immunohistochemical stained with standard streptavidin biotin AP technique . YM14673(1 mg/kg, ip, 30 min prior to occlusion)decreased the degeneration of neurons in the cerebral cortex at 1 h of MCA/CCAs occlusion with 24 h of reperfusion. Most of the pyramidal cells in CA4, and dentate granule cells showed intense c fos protein like immunoreactivity(CFPLI)at 1 h of MCA/CCAs occlusion with 4 h of reperfusion. YM14673 attenuated the c fos protein induction in CA4 regions and dentate gyrus at 1 h of MCA/CCAs occlusion with 4 h of reperfusion. The results show that YM14673 has certain inhibiting function on the expression of c fos in the hippocampal regions post-is-chemia.

Expression of PCNA and Ki67 in Brain Astrocytomas

Zhou Dabiao;Zhao Jizong;Dai Ke

2001, 22(3):  213-215. 

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To elucidate the clinical value of expression of proliferating cell nuclear antigen(PCNA)and Ki67 in brain astrocytomas, the immunostaining of PCNA and Ki67 in 38 astrocytomas were performed by Streptavidin-Peroxidase(SP)method. The result showed the difference of PCNA labeling index(PCNA LI)between tumor grades were statistically significant(P<0.05)except those in group Ⅲ and group Ⅳ. In general,PCNA LI increased with tumor grade. Though total variance of Ki67 LI among tumor grades was remarkable(P< 0.05), there were no significant difference among groupⅠ and Ⅱ and group Ⅲ and Ⅳ. The mean tumor volume had a positive correlation with PCNA LI(r=0.97, P< 0.05). It can be concluded that the expression of PCNA at protein level assessed half quantitatively by immunohistochemistry combined with pathology seem to give reliable information about biological behavior including proliferative potential, growth patterns and histological feather of astrocytomas.

Medication of Traumatic Brain Edema in Rats

Jia Wang;Yu Chunjiang;Wang Fengmei;Chen Fei;Cao Wenjun;Chen Ruifen

2001, 22(3):  216-219. 

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To study the different medication of traumatic brain edema in rats, 54 rats in which the models of traumatic brain edema were induced by Feeney's extradural impact were divided into six groups randomly. Different groups were treated with special drugs and different dosages. The study observed the changes of the blood-brain barrier with Evans Blue and the water content of the brain after the brain injury in rats. Injury areas were observed by pathological techniques in the experiment. The decreases of injury area and the water content of the brain after the brain injury were the most evident in the groups that were treated with large-dosage mannitol. It was proved by the pathological observation that brain injury was the lightest in the asme groups. Curative effect of little dosage mannitol and frusemin used together can last for a long time. Mannitol is the most effective drug in treating acute traumatic brain edema , but little dosage mannitol and frusemin used together is the best choice after mannitol was used solely five to six times.

Antihypertensive Effects of Bisoprolol on Hypertensive Patients

Zhao Xiuli;Hu Dayi;Jia Sanqing;Gao Mingming;Han Xianhua;Xu Zhimin

2001, 22(3):  220-221. 

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To evaluate the antihypertensive effect and safety of bisoprolol, 30 patients with mild to moderate primary hypertension were treated with bisoprolol 5 to 10 mg, once daily for 8 weeks. It were also monitored that changes of blood pressure before and after medicine by 24 h ambulatory blood pressure monitoring. 29 cases were followed up. The total effectiveness was 86.2%. Compared with pre treatment, the decreased SBP and DBP had significant statistical difference. The T/P ratio was >50%. The adverse effect was low, and there was no side effect on the metabolic of cholesterol and glucose . The results shows that bisoprolol is effective and safe in the treatment of mild to moderate primary hypertension.

Effects of Losartan on 24 Hour Ambulatory Blood Pressure in Patients with Essential Hypertension

Xing Xiurong;Hua Qi

2001, 22(3):  222-225. 

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Thirty patients with mild to moderate essential hypertension were treated with losartan(50 mg/d)for 4 weeks. Clinical blood pressure(CBP)and ambulatory blood pressure monitoring(ABPM)were performed at before and after the treatment to compare the mean blood pressure of 24 h, daytime and night, the load of the blood pressure and the circadian rhythm. Results: CBP, the load of blood pressure and the mean blood pressure of 24 h, daytime and night were significantly reduced. No significant difference was found in heart rate. The effective rate dependent CBP was 70.0%. There were significant decreases in every hour blood pressure. There was less effect on night blood pressure, but the circadian rhythm wasn′t significantly changed. The trough to peak ratios for SBP and DBP were more than 70%. The result shows that losartan could significantly and safely decrease the blood pressure. It might maintain normal circadian rhythm of the blood pressure.

Evaluation of Ventricular Function after VVI Pacing with Radionuclide Ventriculography

Yang Honglian;Mo Liying;Wang Xuelian;Du Fenghe

2001, 22(3):  226-227. 

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To evaluate ventricular function of patients after VVI pacing, 20 patients after VVI pacing and 19 controls were studied by gated cardiac blood pool imaging. There was no significant difference in the parameters of systolic function(LVEF?RVEF?LVPER?LV1/3EF?LV1/3ER)between VVI group and control group(P>0.05). There was no difference in the parameters of diastolic function(LVPFR?LV1/3FF)between the two groups either(P>0.05). LV1/3FR of the VVI group was significantly higher than that in the control group(P<0.05). The parameters of left ventricular systolic coordination(PS and LVPHSD)were significantly lower after VVI pacing than the control group(P<0.01). The results suggest that the left ventricular systolic coordination of patients after VVI pacing is lower and ventricular function remains stable.

Relationship between BMI and Prevalence Rate of Hypertension of Middle and Elderly Rural Residents in China

Liu Lei;Zhang Xin;Zhao Wenhua

2001, 22(3):  228-229. 

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To study the relationship between BMI and the prevalence rate of hypertension, the blood pressue, weight and height of 534 people from ruralarea, aged 50～69 years were measured with standardized methods and under strict quality control. The investigated people were grouped by age、sex and BMI. The prevalence rate(55.3%)of hypertension for the obesity group(BMI≥25)was obviously higher than the normal group(BMI=18.5～24.9, 32.3%)and the light body weight group(BMI<18.5, 31.4%, P<0.05)for male. For female, the prevalence rate of hypertension obesity group(56.3%)was obviously higher than that of the normal group(31.3%)and the light body weight group(25.8 %, P< 0.05). Obesity is an important factor resulting in hypertension, and hypertension should be prevented by controlling the body weight.

Clinical Observation for 60 Patients with Type 2 Diabetes Mellitus Treated by Basen

Xu Baorong

2001, 22(3):  230-231. 

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The study observed the curative effects and adverse effects of basen, one of α-glucosidase inhibitors. I have treated 60 patients with type 2 diabetes mellitus for 3 months. FBG and PPG were assessed . The results showed that FBG decreased(P<0.05)and PPG decreased significantly(P<0.01). Abdominal distension and exsufflation were its adverse effects.

Influence of Hemodialyzer Membranes on Apoptosis of Peripheral Blood Mononuclear Cell

Liu Huilan;Wang Ying;Zhang Xiaojie;Zhao Yanchi;Zhang Haiyan;Li Weihong

2001, 22(3):  232-235. 

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This study investigated the influence of hemodialyzer membranes on apoptosis of peripheral blood mononuclear cell. PBMC were seperated from whole blood donated by healthy volunteers . They were incubated in vitro in the presence of either CU membrane or Gambrane membrane . Control cells were incubated in absence of HD membranes. The cells were collected at regular intervals. PBMC apoptosis were evaluated with flowing cetometric analysis. The results indicated that the apoptosis of PBMC in each group increased more obviously than that before the incubation 48 h ago. Apoptosis of CU group was(36.31±1.40)%, significantly higher than other two groups(P<0.05). CU membrane, a bioincompatible HD membrane, can induce the isolated PBMC to apoptosis more easily than others.

Study of Interdigestive Migrating Motor Complex and Plasma Motilin Concentration in Diabetic Gastroparesis

Zhang Tongyan;Wang Yan;Yuan Shenyuan;Ding Yan;Chen Bo;Wan Xiaoping

2001, 22(3):  236-238. 

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The study is to observe the changes of gastric motility and motilin in diabetic gastroparesis(DGP)and to determine the effects of cisapride therapy in DGP. Twelve normals and 32 diabetic patients participated. Basal gastric motility was studied in both groups and plasma motilin(MTL)concentration was studied in each phases of MMC. Thirty-two diabetic patients were equally divided into 2 groups. Cisapride was orally administered in group one while non prokinetic drug was administered in group two as the control. The same examination was carried out in both groups. Results: ① In diabetic patients, abnormal MMC was characterized by absence of phase 3, prolongation of phase 2, decrease of motor index of phase 2 and amplitude of phase 3. ② In the control group, MTL concentration fluctuated in phases of MMC and reached a peak level in late phase 2. The MTL concentrations of patients were significantly higher than that of the control group and had normal cyclic fluctuations of MTL. ③ After cisapride treatment, MTL concentration were reduced significantly, and the contractile activity, amplitudes in phase 3 and motor index of phase 2 were increased significantly. It showed that gastric motility was depressed in diabetes. Abnormal motility in DGP might not be related to MTL deficiency. Cisapride administration in DGP can improve gastric motility and decrease MTL concentration.

Pathogenesis of Myasthenia Gravis Complicated with Epilepsy

Fang Libo;Liu Guangzhi;Xu Xianhao;Chen Weiqun

2001, 22(3):  239-240. 

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This study explored the pathogenesis of myasthenia gravis(MG)complicated wity epilepsy. The study analyzed the clinical manifestation and experimental results of 15 MG patients complicated wity epilepsy. The attack of epilepsy occurred with the aggravation of the clinical symptom of MG and relived with the improvement of MG respectively. The levels of CSF IgG and the CSF IgG 24 h synthetic rate in the group with MG complicated with epilepsy(MG+EPI)were higher than those in the other two groups, the group of MG without EPI(MG-EPI)and the control group. The attack of epilepsy is related with the abnormally synthetic acetylcholine receptor antibody(AchRab)in the central nervous system.

Complications and Death Cause Analysis of 56 Cases of Fatal Epidemic Encephalitis B Treated with Artificial Respirator

Yang Jianguo;Wang Juntao;Lin Xiuyu

2001, 22(3):  241-243. 

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This study analysed complications and death cuases of 56 cases of fatal epidemic encephalitis B treated with artificial respirator. Average age was 19 years.50/56(89%)cases had complications . 48/56(85.7%)cases hadhospital infection.29 cases had bacillus aeruginosus infection. 8 cases had bacillus mucosus capsulatus infection.3 cases had fungus infection. One cases had bacillus coli infection.7 cases had other G⁺ bacteria infection.During the ten days treated with mechanical ventinator 4/56(7.14%)cases had hospital infection. With the days of mechanical ventinator increased there were 48/56(85.7%)cases who had hospital infection. During 20 days with mechanical ventinator 2/56(3.6 %)cases had bacillus aeruginosus infection. After 20 days with mechanical ventinator 27/56(48.2 %)cases had bacillus aeruginosus infection(P< 0.01). 5 cases had pressure injure. 2 cases had hypodermal emphysema. 3 cases had mediastinum emphysema. 20 cases had hemorrhage of degestive tract. Conclusion :The duration of mechanical ventinator was the key to hospital infection. To decrease the time of mechanical ventinator may decrease infection and fatality rate.

Proliferation Effects of bFGF on Rabbit Articular Chondrocyte Culture in Vitro

Feng Mingli;Yong Yimin;Shen Huiliang;Hu Huaijian

2001, 22(3):  244-247. 

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The purpose of this study is to investigate the effect of bFGF on rabbit articular chondrocyte culture in vitro, so that the study could help search a clue of treatment for repair of cartilagious injury. Chondrocytes were isolated from articular cartilage of 1～3 d old New Zealand rabbit. Condrocytes were seeded at different densities of in 10 mL 1640 medium with different concentration of bFGF, to observe the proliferation effects of bFGF on chondrocytes and the best concentration of bFGF for stimulatory proliferation and to measure the amount of incorporation of ³H- thymidine. The amount of chondrocyte increased with increase of concentration of bFGF and the best concentration of bFGF was 10～15 μg/L for stimulatory proliferation. The amount of incorporation of ³H- thymidine was greater in chondrocyte culture grown with bFGF than that tacking bFGF(P<0.05). bFGF exerts a strong effect on proliferation of cultured chondrocytes and the best concentration of bFGF was 10～15 μg/L.

Application of Cholangioscopy in Operation of Bile Duct Stones

Li Hailong;Wang Yan;Yu Wei

2001, 22(3):  248-249. 

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The study investigated the practical value of cholangioscopy in operation of bile duct stones. The study analysed 107 cases undergoing poerations of bile duct stones with cholangioscopy. Before cholangioscopy examination residual stone rate of intrahepatic duct stone and extrahepatic duct stone operation was 94% and 15% respectively. After cholangioscopy examination residual stone rate was 19% and 4% respectively. After the operation,the study used cholangioscopy to remove residual stone of average 3.8 times per person for those without cholangioscopy in operation, but for those with cholangioscopy in operation, only twice per person. Applieation of cholangioscopy in operation of bile duct stones is an effective method for reducing the residual stone rate.

Clinical Effects of Taking Low-dosage Ru486 after Mifepristone Abortion

Wu Qingqing;Zhang Lisong;Gao Jinhua;Ma Huiqing

2001, 22(3):  250-252. 

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The study investigated the effects of taking low-dosage of Ru486 in discharging the uterine remnants and shortening vaginal bleeding time after mifepristone abortion. 235 cases of early pregnancy women were divided into tow groups. 116 cases who took 50 mg Ru486 after blastula discharged was the clinical group. The other 119 cases who did not take any Ru486 was the control group.The difference of the rates of complete abortion and the time of vaginal bleeding between two groups was observed. The rate of complete abortion in the clinical group was higher than that in the control group(P<0.01).The vaginal bleeding time of the clinical group was shorter than that of the control group(P<0.01).The rate of D&C after abortion in the clinical group was significantly lower than that in the control group(P< 0.01). Low-dosage Ru486 can serve to speed up the discharging of uterine remnants to shorten the vaginal bleeding time after mifepristone abortion.

Effect on Endocrine, Bone and Lipoid Metabolism in Women of Reproductive age Undergoing Unilateral Ovariectomy

Jia Xiaofang;Guo Xiaorong;Hu Suisu;Wang Shufen;Zhu Xian

2001, 22(3):  253-254. 

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The study eavluated the effect on bone and lipoid metabolism in women of reproductive age undergoing unilateral ovariotomy. A postoperative follow-up study was performed in 21 cases for three years after operation and twenty normal women were enrolled in control group. There was no significant difference in bone density, biochemical markers of bone metabolism or the levels of plasma E₂, and FSH between two the groups. However, the concentration of plasma T was markedly higher in the study group. And TC and apoB levels were higher in the study group. It seems there is no effect on bone metabolism in women with unilateral ovarotomy. After operation, lipoid metabolism is changed.

Analysis on 22 Ecchordosis Physaliphora Cases Related to Otorhinolaryngology

Yang Lihua;Wang Qi

2001, 22(3):  255-256. 

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Eighty cases of ecchordosis physaliphora were analyzed, of whom 22 cases were found to have symptoms of ear-nose-throat, which account for 27.5% of the total. There were 13 cases with initial symptoms of ear-nose-throat, which accounted for 16.25%. This indicates that ecchordosis physaliphora may cause ear-nose-throat symptoms. Clinical data analysis reveals that the symptoms of ear-nose-throat caused by ecchordsis physaliphora often last for a long time. It is difficult to make a definite diagnosis for those patients with tinnitus, nasal obstruction and problems on laryngeal part of pharynx. For those young patients who have not intracranial hypertension, and no improvement on ear-nose-throat symptoms after treatment, doctors should give them a CT/MRI examination in time, so as to rule out the possibility of suffering from ecchordosis physaliphora.