Table of Content

15 July 2000, Volume 21 Issue 3

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论著

Culture of Bovine Chromaffin Cells and Determination of Dopamine Secreted

Lü Jie;Zhang Jinlu;Zhao Chunli;Duan Chunli;Xu Qunyuan

2000, 21(3):  185-186. 

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The morphological changes of cultured bovine chromaffin cells were observed and the dopamine secreted was determined by HPLC to explore the feasibility of gene therapy with adrenal medulla cell transplantation. Results: The chromaffin cells in suspension culture, aggregated in round shape all the time and could secrete dopamine, and were still alive after resuscitation from cryopreservation; the adhesion cells showed round shape first, then stretched out short processes, turned in to shapes as triangle, fusiform, and polygon et al and restored to round shape after a week of culture. The HPLC results showed that the dopamine content arrived at the peak at about 5～6 days of culture. Adrenal chromaffin cell is a valuable source that can be used for gene therapy.

MRI Finding of Parkinsonizm in Primate after MPTP Exposure

Gu Yebing;Li Kuncheng;Yao Xinyu;Zhang Jinlu;Liu Yujun;Lu Qiang;Xu Qunyuan

2000, 21(3):  187-189. 

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Five primate models for Parkinsonizm, which was established by 1-methyl-4-pheny-1,2,3,6-tetrahydropyridine(MPTP) injecting through unilateral internal carotid after 6 weeks, were scanned by MR imaging. MRI demonstrated that the area of the substantia nigra decreased and there was foci of increased signal intensity in homolateral on T₂-weighted images. Histological examination showed a loss of tyrosine hydroxylase immunoreactive neurons and pervasive gliosis in the ventral midbrain area on the injection side by Grino staining. The results manifested that MRI can be a modality to evaluating the primate modal of Parkinsonnism after MPTP exposure.

Effects of Traditional Chinese Compound 962 on Choline Acetyltransferase and Acetylcholinesterase in Cerebral Cortex of Aged Rats

Li Hailing;Li Lin;Li Bin;Ye Cuifei;Li Zongxin;He Shida;Zuo Pingping

2000, 21(3):  190-192. 

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In order to determine the effect of the compound 962 capsule on the choline acetyltransferase and acetylcholinesterase in cerebral cortex of aged rats, and to probe the anti agedness mechanism from the view of choline energy nerve. Male and female aged rats(22-month old and 24-month old) were classified into five groups young——control group, old model group, piracetem group (0.56 g/kg), 962 middle dose group (0.9 g/kg) and 962 high dose group (1.8 g/kg). All test drug was administrated for 1～2 months by ig. The choline acetyltransferase and acetylcholinesterase activity were measured by Fonnun and hydroxylamine colorimetry method. Results: Compound 962 improved the activity of choline acetyltransferase (P< 0.05), but it had little effect on the acetylcholinesterase in cerebral cortex of aged rats. This indicated that by affecting the central nervous system in aged rats, compound 962 capsule could improve the aging process.

The Relationship between Blastocyst Implantation and Active Proteins within Granulated Metrial Gland Cells

Weng Jing;Xu Qing;Liang Yuanjing;Lu Xin;Shi Xiaolin;Zhu Dingshou

2000, 21(3):  193-194. 

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Twenty mice in 3 days of gestation were divided into 2 groups. Ten mice were subcutaneously injected with proteins derived from the isolated granulated metrial gland cells (as experimental group), the others with PBS (as control group). All mice were killed on the 5th day after injection. The numbers of pregnant mice and the implanted blastocysts were counted. Nine out of ten mice in experimental group were pregnant with totally 112 implanted blastocysts, whereas 7 out of ten mice with 87 implanted blastocysts in control group. The results indicate that proteins derived from the isolated GMG cells may promote the implantation of blastocyst.

Study on Effects of Combined Application of Propranolol and Nifedipine on Portal Hemodynamics in Experimental Cirrhotic Rats

Yang Dongxu;Hu Xiaomin;Ding Huiguo

2000, 21(3):  195-197. 

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The research on the effects of sigle and combined application of propranolol and nifedipine on portal hemodynamics in experimental cirrhotic rats were carried out by testing the rats′ average artery pressure, portal pressure and portal bloodstream. The results demonstrated that propranolol and nifedipine, whether used singly or in combination, could both decrease the portal pressure and rate of portal bloodstream (P<0.05 or P<0.01) in the rats, but neicher had apparent effect on the average artery pressure, and furthermore, there were no apparent diferences on the effects between the two drugs. In conclusion, combined use of propranolol and nifedipine has a distinct improvement on portal hemodynamics in experimental cirrhotic rats, whose effect is approximately equal to either of the drugs used singly.

Transmyocardial Laser Revascularization in Senile Patients with High Risk

Qu Zheng;Zhang Zhaoguang;Yu Jianbo;Ye Jianguang;Sun Yanqing;Ma Lin′an;Xu Yi

2000, 21(3):  198-200. 

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To report the perioperative characteristics in transmyocardial laser revascularization (TMLR) in senile patients .TMLR was performed on the beating heart with high power CO₂ laser (the Heart Laser, ^TMPLC. Inc.)in 33 high risk cases. Transesophageal echocardiography (TEE) showed the average number of channels was 22±5. The follow up ranged from 1 to 24 months was made to assess angina class and myocardial perfusion. 2 patients died of acute myocardial infarction and one died of cardiac shock. Some people suffered related complications such as arrhythmia, pneumothorace, etal. The follow-up included 26 patients. The angina status were improved in 23(88%) patients. ²⁰¹Tl-SPECT showed a remarkable improvement in reversible ischemia in 16(62%) follow up patients. TMLR for patients with high risk is safe and effective. Monitor during operation is very important for prognosis.

Effects of Fluvastatin on Serum Lipid and its Relationship with Intimal Hyperplasia in the Hyperlipidemic Model of Rabbit after Iliac Artery Endothelial Denudation

Li Dongbao;Shen Luhua;Xie Miaorong;Chen Hui

2000, 21(3):  201-203. 

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To investigate the effects of fluvastatin on blood lipid in inhyperlipidemic model of rabbit after iliac artery endothelial denudation and the relationship between serum lipid and intimal thickening in the study, the New Zealand white rabbits were randomized into three groups: drug treatment group(n=28),non drug treatment group(n=28)and control group(n=7). The atherosclerotic lesions were developed by endothelial denudation with balloon catheter and high dosage of cholesterol diet at the same time. The experimental animals were killed and iliac arteries were removed at 1、2、4、8 weeks after endothelial injury respectively and bloodsamples were taken at the same time. By the methods of routine HE staining, blood lipid measure and image analysis system, observed the effects of fluvastatin on the level of serum TC、TG、LDL-C and the thickness of intima and media,intima/media(I/M) ratio and the relationship between serumlipid and intimal thickening. The level of TC、TG、LDL-C in drug treatment group was remarkably lower than that in non drug treatment group(P<0.05). The thickness of neointima and I/M in drug treatment group was much thinner than that in non drug treatmeant group. Coefficient of linear correlation between intimal thickness and TC、TG、LDL-C were 0.911,0.892,0.942 (P< 0.01) respectively.Fluvastain can lower blood lipid apparently, which may relate its effect to inhibiting excessive thickening of vascular intima.

Clinical Analysis of Heart Rate Variability in Patients with Acute Head Injury

Du Fenghe;Liu Jin;Zhang Tong;Li Mingyue;Zhao Jizong;Fu Zhuang

2000, 21(3):  204-205. 

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To study the variation of heart rate variability and its association with QT interval dispersion in patients with acute head injury, 24 hour ambulatory electrocardiographs collected in 60 patients with in 72 h after acute head injury and 30 control subjects were analyzed in the respect of the heart rate variability of Time Domain Methods by using the DMS software. Result:①All parameters of heart rate variability were reduced in the patients with acute head injury(P<0.01).② QT interval dispersion(QTd) positively correlated with the standard deviation of the RR intervals(SDNN) in the severed patients (P<0.05).It suggests that the variation of HRV and QTd may serve as a predictor in the severed arrhythmia of patients after head injury.

Relationship between Specific HP Strain Infection with CagA Seropositive and Gastroduodenal Diseases

Zhu Yuqun;Wang Yanbin;Li Xinxin;Zhang Lei;Yang Zhaoxu

2000, 21(3):  206-208. 

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In order to assess the relationship between different HP bacteria types and their pathogenicity,investigated 1 674 patients who underwent gastroscopy and 100 blood donors. HP infection was diagnosed by at least 2 positive results from PCR, histology (Giemsa staining), or rapid urease test. Serum CagA IgG antibodies were measured by ELISA. It was found that total prevalence of CagA seropositive was 49.70% in patients and 38% in controls (P<0.05). The frequency of CagA antibodies were significantly higher in DU cases than that of in GU,GC and CG ones(P<0.01). In active and severe degree of CG, anti-CagA presence was more common(P<0.01). However, no difference was found in anti-CagA positive rate among GC, CAG, IM, AH and normal groups. These data indicated that specific HP strain with CagA seropositive was associated with the degree of damages and active inflammation on gastroduodenal mucosa.

Protective Effect of Abelmosehus Manihot on Rats Renal Tubular with Nephrotic Syndrome

Yin Lianfang;Liu Lu;Gong Yuxiang;Pu Jiakang

2000, 21(3):  209-211. 

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To investigate the protective effects of Abelmosehus manihot (Am) on rat renal tubular with nephrotic syndrome, the nephrotic rat model was made by tail vein injection of adriamycin 5mg/kg. Am was given and changes of urinary type Ⅲ collagen(C-Ⅲ),N-acetyl-β-D-glucosa minidase(NAG)and β₂ microglobulin(β₂-MG) were observed. It was shown that the excretion of urinary C Ⅲ,NAG and β₂-MG in nephrotic rats were significantly increased as compared with those of the normal control group (P< 0.01). In the group treated with Am, the excretion of urinary C-Ⅲ,NAG and β₂-MG did not increase obviously . Am exerted strong protective effects on rats renal tubular damage with adriamycin induced nephrotic syndrome.

Clinical Significance and Variation of Fibrinolysis before and after Dialysis in Patients with Uremia

Gao Guojing;Cheng Cuiling;Jia Qiang;Liu Rongkun

2000, 21(3):  212-213. 

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For investigating the variation of fibrinolysis in patients with uremia and the influence of dialysis on fibrnolysis, Plg, t-PA, PAI and D-D were examined in 20 patients with uremia and 13 healthy controls. It was found that t-PA was sousiderably higher in patients with uremia than in controls (P<0.01), D-D decreased and PAI and Plg increased, and there were no any differences before and after dialysis in patients with uremia. It was suggested that variation of fibrinolysis in patients with uremia is not influenced by dialysis.

Effect of Calcium Channel Blocker on the Expression of Cyclooxygenase-2 in Rats Focal Cerebral Ischemia

Long Jie;Zhang Zaiqiang;Lu Dunyue

2000, 21(3):  214-216. 

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To explore the potential impact of COX 2 on cerebral ischemic injurious mechanism,the present studies examined the influence of calcium channel blocker on the expression of COX 2,which is the key enzyme in arachidonic acid metabolism. The model of middle cerebral artery occlusion (MCAO) and immunohistochemistry staining were used to compare the expression of COX 2 protein in cerebral ischemic group with that in nimotop treatment group. There were no immunochemistry positive staining cells found among either the sham operation subgroup or the subgroup after MCAO for 1 and 4 h; however, positive staining cell began to acumulate after MCAO for 12 h. The positive cells in nimotop group were significantly higher than that in group without intervention: 12 h subgroup 56±11/mm 2 vs 45±18/mm 2(P< 0.05); 24 h subgroup 117±16/mm 2 vs 69±14/mm 2(P<0.01); 48 h subgroup 81±14/mm 2 vs 41±13/mm 2(P<0.01). The positive staining only occurred in intact neurons surrounding the core of the infarction. The infarcted area in nimotop group were significantly less than that in group of MCAO for 24 h[(28.308±3.959)mm 2 vs (46.780±6.167)mm 2, P<0,01). It indicated that Calcium channel blocker can effectively ameliorate experimental cerebral ischemic injury. The characteristics of delayed expression of COX 2 surrounding the core of infarct and the up regulation of COX 2 by calcium channel blocker, suggested that COX 2 may play a active role in the protective mechanism after ischemic cerebral injury.

A Analysis of Risk Factors and Onset of Age of Stroke

Xie Shuping;Zhao Lijie;Liang Baohua;Liu Qing

2000, 21(3):  217-220. 

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The objiective of the study is to observe the changing in risk factors and onset age of stroke in recent years. 2 154 cases of patients from 1995 to 1996 were collected, and 10 547 cases of data from review of cases in past 25 years (1972～1996) were compared. The percent of patients younger than 51 is 16.32% for 1972～1976, 22.72% for 1992～1996 respectevely. The percent age of patients younger than 41 is 1.29% 1972～1976, 7.83% for 1992～1996. Risk factors of patients are hypertension (81.1%), hyperlipoidemia(68.4%), hyperglycosemia(54.4%), and the occurrence of cerebrovascular disease tends to transit to younger group of people.

Evaluating the Effect of Sandostatin for Prevention and Treatment of Stress Ulceration in Acute Severe Brain Injury Patients

Wang Rong;Wang Dejiang;Zhao Yuanli;Fu Zhuang

2000, 21(3):  221-223. 

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To evaluate effect of Sandostatin in prevention and treatment of stress ulceration for acute severe brain injury patients, 30 patients with acute severe brain injury (GCS 3～8) were admitted into this study. Besides neurosurgery routine treatments, they received sandostatin during the first 7 days (0.1 mg, per 8 h) and were observed in the next 3 weeks. The other 86 patients with acute severe brain injury, as the controls, just received histamine H 2 antagonist treatment. Only 4 (13.3%) of the 30 patients in sandostatin group suffered from upper gastrointestinal hemorrhage, and all happened in the first 24 h and were controlled within 1 d. In the control group, 16 patients (18.6%) suffered from upper gastrointestinal hemorrhage(P<0.05). Conclusion: sandostatin is effective for prevention and treatment of the stress ulceration in acute sever brain injury patients.

The Clinical Analysis of 317 Cases of Postpartum Hemorrhage

Liu Yuhuan;Jiang Guiying

2000, 21(3):  224-225. 

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To investigate correlative factor and prevention and treatment of postpartum hemorrhage, 317 cases of postpartum hemorrhage were reviewed. The incidence of postpartum hemorrhage, during January, 1986 to June, 1999 ,were 1.53%(317/20 768). The chief reason of postpartum hemorrhage was uterine inertia, taking up 53.31%(169/317). The incidence that caesarean section occurred postpartum hemorrhage were 2.41%(151/6 278) of the cases, which was higher than vaginal delivery(P<0.01). Conclusion: Reducing the uterone inertia and caesarean seclion is the key to reduce the incidence of postpartum hemorrhage.

Experimental Study of Self Expandable Metal Stents Inserted in Trachea of Piglets

Ai Lin;Dai Jianping;Guo Qiyong;Liu Zhaoyu

2000, 21(3):  226-229. 

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To study situation of growing trachea inserted with self expandable metal stents and provide the base of treating stenosis of trachea in childhood with the stents, stents of different tension being inserted into tracheas of two groups of piglets. The symptoms of the animals, the changing diameters of tracheas and stents, and the appearance of gross specimen and histopathology were observed. All, excep one, the animals had little or no symptoms. Stents inserted migrated in two animals. No trachea stenosis happened in low tension stent group. Inflammation existing in trachea of low tension stent group was milder than that of middle tension stent group. There is possibility that low tension and large diameter self expandable metal stents can be used to treat the stenosis of trachea in childhood.