Journal of Capital Medical University ›› 2009, Vol. 30 ›› Issue (2): 133-136.

• 肾病学专题 • Previous Articles     Next Articles

Role of Endogenous H2S in the Accumulation of Extracellular Matrix of the Diabetic Nephropathy

HUANG Wen, LI Xiao-lu, CHEN Shen, ZHANG Hong, YANG Min   

  1. Department of Nephrology, Tongren Hospital, Capital Medical University
  • Received:2009-01-18 Revised:1900-01-01 Online:2009-04-21 Published:2009-04-21

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Abstract: Objective To explore changes of fibronectin(FN) in the kidney tissues of diabetic nephropathy)(DN) rats, and to study the role of endogenous H2S in the pathological development of diabetic nephropathy. Methods Twenty-eight rats(male, Sprague-Dawley) were randomly divided into four groups: control group, diabetic nephropathy group, DN+NaHS(H2S donor) group and DN+DL-propargylglycine(PAG, H2S synthetase inhibitor) group. Relative location and expression of FN in kidney tissues were measured respectively by Western blotting and immunohistochemistry. Results FN was positive in both the intraglomerular mesangial region and the extracellular matrix(EMC) in kidney tissues of DN group, and its expression level increased significantly. Western blotting: DN group (48 896±779) vs control group(38 531±902), P<0.01. Compared with that of DN group and DN+PAG group, NaHS significantly inhibited the over-expression of FN, Western blotting: DN+NaHS group(42 737±1 196) vs DN group(48 896±779) and DN+PAG group(48 252±1 068), P<0.01. Conclusion Endogenous H2S can alleviate the pathological progression of DN by inhibiting the accumulation of ECM.

Key words: hydrogen sulfide, diabetic nephropathy, fibronectin, Western blotting

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