Expression and corticosteroid inhibition of arachidonate 15-lipoxygenase in chronic rhinosinusitis with nasal polyps

doi:10.3969/j.issn.1006-7795.2017.05.011

Journal of Capital Medical University ›› 2017, Vol. 38 ›› Issue (5): 683-687.doi: 10.3969/j.issn.1006-7795.2017.05.011

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Expression and corticosteroid inhibition of arachidonate 15-lipoxygenase in chronic rhinosinusitis with nasal polyps

Yan Bing¹, Wang Yang¹, Fan Erzhong¹, Li Ying¹, Qi Sihan², Chu Xiaohan², Wang Chengshuo², Zhang Luo^1,2

1. Beijing Institute of Otorhinolaryngology, Beijing Key Laboratory of Nasal Diseases, Key Laboratory of Otorhinolaryngology Head and Neck Surgery, Ministry of Education of China, Beijing 100005, China;
2. Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China

Received:2017-07-07 Online:2017-09-21 Published:2017-10-18
Supported by:
This study was supported by National Natural Science Foundation of China (81630023, 81400444, 81470678, 81420108009), Beijing Municipal Administration of Hospitals' Mission Plan (SML20150203), Beijing Municipal Administration of Hospitals' Youth Programme (QML20150202), Beijing Clinical Research Foundation (Z141107002514122), Capital's Funds for Health Improvement and Research (2016-1-2052), Talents Project of Beijing Municipal Human Resources and Social Security Bureau (2016).

Abstract

Abstract: Objective To detect the expression of arachidonate 15-lipoxygenase (ALOX15) in eosinophilic chronic rhinosinusitis with nasal polyps (ECRSwNP), and nonesinophilc chronic rhinosinusitis with nasal polyps (nonECRSwNP). Moreover, the effect of corticosteroid on ALOX15 expression was evaluated. Methods Samples were socked in RNA later and embedded in paraffin were acquired from library of Beijing Tongren Hospital. Real-time PCR and immunohistochemistry (IHC) were applied for mRNA quantification and protein distribution of ALOX15 in ECRSwNP, nonECRSwNP and healthy control group respectively.ALOX15 mRNA level of ECRSwNP samples pre-and post-corticosteroid treatment were detected. Results LOX15 mRNA level and protein expression were found increased significantly in ECRSwNP compared with nonECRSwNP and healthy control (both P=0.000). There was no significant difference between nonECRSwNP and healthy control (P=0.594, P=0.550). ALOX15 protein localized in a majority of cells including epithelia, plasma cells,neutrophils and eosinophils.One-week oral corticosteroid could decrease ALOX15 mRNA and protein expression in ECRSwNP significantly (P=0.004, P=0.024). Conclusion ALOX15 is upregulated in ECRSwNP compared with nonECRSwNP and control groups. Corticosteroid treatment could inhibit ALOX15 expression in ECRSwNP.

Key words: nasal polyps, chronic rhinosinusitis, arachidonate 15-lipoxygenase

CLC Number:

R765.4

Yan Bing, Wang Yang, Fan Erzhong, Li Ying, Qi Sihan, Chu Xiaohan, Wang Chengshuo, Zhang Luo. Expression and corticosteroid inhibition of arachidonate 15-lipoxygenase in chronic rhinosinusitis with nasal polyps[J]. Journal of Capital Medical University, 2017, 38(5): 683-687.

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Expression and corticosteroid inhibition of arachidonate 15-lipoxygenase in chronic rhinosinusitis with nasal polyps

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